VCAM1 Antibody (YA5098)
(Synonyms: VCAM1; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD antigen CD106)VCAM1 Antibody (YA5098) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to VCAM1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, FC, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:5000-10000 | 1:1000 | 1:200 | 1:100 | 1-2μg/sample | 1:1,000 |
Product Details
VCAM1 Antibody (YA5098) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to VCAM1.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 81 kDa
Synthetic peptide within Human VCAM1 aa 690-739 / 739.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
VCAM1 cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation[1][2][3][4].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Secreted
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Expression
Tissue_specificity:It is expressed on inflammatory vascular endothelial cells, as well as macrophage-like cells and dendritic cells in normal and inflammatory tissues.
Induction:By pro-inflammatory cytokines, including TNFalpha, and also by ROS, oxidized low density lipoprotein, high glucose concentration, toll-like receptor agonists, and shear stress -
Isoforms & Post-Translational Modification
P19320 has 3 isomers: P19320-1: 81276 Da (predicted); P19320-2: 71255 Da (predicted); P19320-3: 74346 Da (predicted).
Cleaved by the metalloproteinase ADAM17 to generate the soluble form;Sialoglycoprotein;Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination; leading to proteasomal degradation -
SwissProt ID
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Synonyms
VCAM1; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD antigen CD106
Documentation
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