RNF26 Antibody (YA9411)
(Synonyms: ring finger protein 26, RNF26)
Based on 1 Customer Validation
RNF26 Antibody (YA9411) is a Mouse-derived and non-conjugated IgG3 monoclonal antibody, targeting to RNF26.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
RNF26 Antibody (YA9411) is a Mouse-derived and non-conjugated IgG3 monoclonal antibody, targeting to RNF26.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 48 kDa
OMIM: 606130
A synthesized peptide derived from human RNF26.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
RNF26 is an E3 ubiquitin-protein ligase that plays a key role in endosome organization by retaining vesicles in the perinuclear cloud. Acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the ubiquitin conjugating enzyme UBE2J1. Ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Also acts as a regulator of type I interferon production in response to viral infection by mediating the formation of 'Lys-11'-linked polyubiquitin chains on TMEM173/STING, leading to stabilize TMEM173/STING. Also required to limit type I interferon response by promoting autophagic degradation of IRF3[1][2][3][4].
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Subcellular Localization
Endoplasmic reticulum membrane
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Expression
Tissue_Specificity: Ubiquitous. Up-regulated in several cancer cell lines. -
Isoforms & Post-Translational Modification
RNF26 has an amino acid length of 433, molecular weight is 47737 Da.
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Subunit
Interacts with INCA1.
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SwissProt ID
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Synonyms
ring finger protein 26, RNF26
Documentation
[1]. Jongsma ML, et al. An ER-Associated Pathway Defines Endosomal Architecture for Controlled Cargo Transport. Cell. 2016 Jun 30;166(1):152-66. [Content Brief]
[2]. Cremer T, et al. The ER-embedded UBE2J1/RNF26 ubiquitylation complex exerts spatiotemporal control over the endolysosomal pathway. Cell Rep. 2021 Jan 19;34(3):108659. [Content Brief]
[3]. Qin Y, et al. RNF26 temporally regulates virus-triggered type I interferon induction by two distinct mechanisms. PLoS Pathog. 2014 Sep;10(9):e1004358. [Content Brief]
[4]. Fenech EJ, et al. Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling. Elife. 2020 Jul 2;9:. [Content Brief]
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