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Galectin-3 Antibody (YA5440)

(Synonyms: LGALS3; MAC2; Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen)
Customer Review

Based on 1 Customer Validation

Galectin-3 Antibody (YA5440) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Galectin-3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
  • Galectin-3 Antibody (YA5440) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:2000-5000 1:100-200 1:50-200 1:5000-20000

Product Details

Description

Galectin-3 Antibody (YA5440) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Galectin-3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Protein

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
    Immunohistochemical analysis of paraffin-embedded human placenta using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
    Immunohistochemical analysis of paraffin-embedded human lymph node using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Galectin-3 is a β-galactoside-binding lectin that regulates cell adhesion, activation, migration, signaling, apoptosis, immune reactions, and neoplastic transformation[1][2]. Mechanistically, its conserved carbohydrate-recognition domain binds β-galactosides, while its intrinsically disordered N-terminal region enables multisite self-association and functional multivalency[1][2]. In inflammation, galectin-3 acts across acute inflammation, chronic inflammation, and tissue fibrogenesis, making it relevant to fibrosis-oriented experimental design[3]. In lung fibrosis models, galectin-3 deficiency or pharmacological inhibition reduced TGF-β1-driven epithelial-mesenchymal transition, myofibroblast activation, and collagen production[4]. More recent human lung fibroblast and IPF lung-slice data show that extracellular galectin-3 promotes integrin-mediated TGF-β1 activation and interacts with αv integrins and TGFβRII in a glycosylation-dependent manner[5]. Compared with related isoforms, galectin-3 is distinguished as the only vertebrate chimera-type galectin, containing one CRD linked to a long proline- and glycine-rich N-terminal domain[1]. For experimental applications, galectin-3 inhibitors that target the carbohydrate-recognition domain blocked integrin colocalization and reduced fibrotic mediators in IPF-derived lung tissue models[5]. In patients with idiopathic pulmonary fibrosis, inhaled TD139 showed target engagement and supported galectin-3 inhibition as a translational research approach[6].- Isoform distinction: galectin-3 differs structurally from prototype and tandem-repeat galectins through its chimera architecture[1]. - Research application: galectin-3 inhibition supports fibrosis studies involving TGF-β1 activation, integrins, and IPF models[4][5][6].

  • Subcellular Localization

    Cytoplasm; Nucleus; Secreted

  • Expression


    Tissue_specificity:This gene is mainly expressed in colonic epithelial cells, and is also abundant in activated macrophages and expressed in the fetal membranes.

  • Subunit

    Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM (PubMed:24945728). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, ZFTRAF1 and UACA. Interacts with TRIM16; this interaction mediates autophagy of damage endomembranes. Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (PubMed:32272059)

  • SwissProt ID

    P17931

  • Gene ID
    3958 [NCBI]
  • Synonyms

    LGALS3; MAC2; Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen

References

[1]. Dumic J, et al. Galectin-3: an open-ended story. Biochim Biophys Acta. 2006 Apr;1760(4):616-35. [Content Brief]

[2]. Lin YH, et al. The intrinsically disordered N-terminal domain of galectin-3 dynamically mediates multisite self-association of the protein through fuzzy interactions. J Biol Chem. 2017 Oct 27;292(43):17845-17856. [Content Brief]

[3]. Henderson NC, et al. The regulation of inflammation by galectin-3. Immunol Rev. 2009 Jul;230(1):160-71. [Content Brief]

[4]. Mackinnon AC, et al. Regulation of transforming growth factor-β1-driven lung fibrosis by galectin-3. Am J Respir Crit Care Med. 2012 Mar 1;185(5):537-46. [Content Brief]

[5]. Calver JF, et al. Defining the mechanism of galectin-3-mediated TGF-β1 activation and its role in lung fibrosis. J Biol Chem. 2024 Jun;300(6):107300. [Content Brief]

[6]. Hirani N, et al. Target inhibition of galectin-3 by inhaled TD139 in patients with idiopathic pulmonary fibrosis. Eur Respir J. 2021 May 27;57(5):2002559. [Content Brief]

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Galectin-3 Antibody (YA5440) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Galectin-3 Antibody (YA5440) Product Image
    1/16
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
  • Galectin-3 Antibody (YA5440) Product Image
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
  • Experimental Validation Results for Galectin-3 Antibody (YA5440)
  • Galectin-3 Antibody (YA5440) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Galectin-3 Antibody (YA5440) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node using Transcription Factor E3 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85748, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Galectin-3 Antibody (YA5440) Product Image IHC-P
  • Galectin-3 Antibody (YA5440) Product Image IHC-P