Orexin Receptor 1 Antibody
(Synonyms: Orexin/Hypocretin receptor type 1, Hypocretin receptor type 1, Orexin receptor type 1, Ox-1-R, Ox1-R, Ox1R, HCRTR1)Orexin Receptor 1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Orexin Receptor 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
Orexin Receptor 1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Orexin Receptor 1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 47 kDa
Synthetic peptide corresponding to the center region of human Orexin Receptor 1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Orexin Receptor 1 is a G protein-coupled receptor that binds the neuropeptide orexin-A with high affinity, and orexin-B with lower affinity, two peptides derived from a common precursor, prepro-orexin. Its activity is mediated via a G(q)-protein-coupled pathway, which activates the phosphatidylinositol-calcium second messenger system in response to orexin-A binding. In addition to G(q)-mediated signaling, orexin-A stimulation also promotes beta-arrestin recruitment, leading to receptor internalization. Plays a significant role in the regulation of food intake (By similarity)[1][2][3].
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Subcellular Localization
Cell membrane
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Isoforms & Post-Translational Modification
Orexin Receptor 1 has an amino acid length of 425, molecular weight is 47536 Da.
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SwissProt ID
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Synonyms
Orexin/Hypocretin receptor type 1, Hypocretin receptor type 1, Orexin receptor type 1, Ox-1-R, Ox1-R, Ox1R, HCRTR1
Documentation
References
[1]. Hellmann J, et al. Structure-based development of a subtype-selective orexin 1 receptor antagonist. Proc Natl Acad Sci U S A. 2020 Jul 28;117(30):18059-18067. [Content Brief]
[2]. Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85. [Content Brief]
[3]. Milasta S, et al. The sustainability of interactions between the orexin-1 receptor and beta-arrestin-2 is defined by a single C-terminal cluster of hydroxy amino acids and modulates the kinetics of ERK MAPK regulation. Biochem J. 2005 May 1;387(Pt 3):573-84. [Content Brief]
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