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Cat. No.: HY-P87706

Shh Antibody (YA7391)

Shh Antibody (YA7391) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Shh.

For research use only. We do not sell to patients.

Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Price

Stock

Quantity

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	IHC-P IHC-P: Immunohistochemistry-Paraffin	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	ELISA ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio	1:200-1:1000	1:2000-1:10000	1:200-1:1000	1:5000-1:20000

Description

Shh Antibody (YA7391) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Shh.

Host Rabbit
Clonality Monoclonal,Recombinant
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 27 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 50 kDa

Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Appearance

Liquid
Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

Shh is a The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the endoplasmic reticulum. Once cleaved, ShhC is degraded in the endoplasmic reticulum|The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO^[1]^[2].
Subcellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane,Secreted,Cell membrane
Isoforms & Post-Translational Modification

Q15465: 462 amino acids, molecular weight 49607 Da.
Subunit

Multimer
SwissProt ID

Q15465