Agrin Antibody
(Synonyms: Agrin)Agrin Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Agrin.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
Agrin Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Agrin.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 217, 205 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 217 kDa
Synthetic peptide corresponding to the C-term region of human Agrin.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Agrin is a Depending on alternative splicing and post-translational modifications, it has a role in different processes, including neuromuscular junction formation and maintenance, and regulation of neurite outgrowth (By similarity). Also involved in positive regulation of cartilage formation through alpha-dystroglycan binding and up-regulation of SOX9[1].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix
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Expression
Tissue_Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues. -
Isoforms & Post-Translational Modification
Agrin has 7 isoforms, O00468-1: amino acid length is 2068, molecular weight is 217320 Da (predicted); O00468-2: amino acid length is 1964, molecular weight is 205631 Da (predicted); O00468-3: amino acid length is 2049, molecular weight is 215345 Da (predicted); O00468-4: amino acid length is 2060, molecular weight is 216454 Da (predicted); O00468-5: amino acid length is 2057, molecular weight is 216211 Da (predicted); O00468-6: amino acid length is 2045, molecular weight is 214846 Da (predicted); O00468-7: amino acid length is 2064, molecular weight is 216820 Da (predicted).
Contains heparan and chondroitin sulfate chains and alpha-dystroglycan as well as N-linked and O-linked oligosaccharides. Glycosaminoglycans (GAGs), present in the N-terminal 110 kDa fragment, are required for induction of filopodia in hippocampal neurons. The first cluster (Gly/Ser-rich) for GAG attachment contains heparan sulfate (HS) chains and the second cluster (Ser/Thr-rich), contains chondroitin sulfate (CS) chains. Heparin and heparin sulfate binding in the G3 domain is independent of calcium ions. Binds heparin with a stoichiometry of 2:1. -
Subunit
Monomer (By similarity).
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SwissProt ID
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Synonyms
Agrin
Documentation
References
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