Agrin Antibody

Secreted, extracellular space, extracellular matrix

Tissue_Specificity: Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.

Agrin has 7 isoforms, O00468-1: amino acid length is 2068, molecular weight is 217320 Da (predicted); O00468-2: amino acid length is 1964, molecular weight is 205631 Da (predicted); O00468-3: amino acid length is 2049, molecular weight is 215345 Da (predicted); O00468-4: amino acid length is 2060, molecular weight is 216454 Da (predicted); O00468-5: amino acid length is 2057, molecular weight is 216211 Da (predicted); O00468-6: amino acid length is 2045, molecular weight is 214846 Da (predicted); O00468-7: amino acid length is 2064, molecular weight is 216820 Da (predicted).含有硫酸乙酰肝素链、硫酸软骨素链、α-肌营养不良蛋白聚糖，以及 N 连接和 O 连接寡糖。存在于 N 端 110 kDa 片段中的糖胺聚糖(GAG)是诱导海马神经元丝状伪足所必需的。第一个 GAG 附着簇(富含 Gly/Ser)含有硫酸乙酰肝素(HS)链，第二个 GAG 附着簇(富含 Ser/Thr)含有硫酸软骨素(CS)链。G3 结构域与肝素和硫酸肝素的结合不依赖钙离子。其与肝素结合的化学计量比为 2:1，与唾液酸结合的化学计量比为 1:1，且结合过程需要钙离子参与(根据相似性推断)Agrin 存在 7 个异构体，O00468-1：氨基酸个数为 2068 个，分子量为 217320 Da (预测)；O00468-2：氨基酸个数为 1964 个，分子量为 205631 Da (预测)；O00468-3：氨基酸个数为 2049 个，分子量为 215345 Da (预测)；O00468-4：氨基酸个数为 2060 个，分子量为 216454 Da (预测)；O00468-5：氨基酸个数为 2057 个，分子量为 216211 Da (预测)；O00468-6：氨基酸个数为 2045 个，分子量为 214846 Da (预测)；O00468-7：氨基酸个数为 2064 个，分子量为 216820 Da (预测)。Contains heparan and chondroitin sulfate chains and alpha-dystroglycan as well as N-linked and O-linked oligosaccharides. Glycosaminoglycans (GAGs), present in the N-terminal 110 kDa fragment, are required for induction of filopodia in hippocampal neurons. The first cluster (Gly/Ser-rich) for GAG attachment contains heparan sulfate (HS) chains and the second cluster (Ser/Thr-rich), contains chondroitin sulfate (CS) chains. Heparin and heparin sulfate binding in the G3 domain is independent of calcium ions. Binds heparin with a stoichiometry of 2:1. Binds sialic acid with a stoichiometry of 1:1 and binding requires calcium ions (By similarity)

Monomer (By similarity).

O00468

Agrin