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RIP3 Antibody (YA6043)

(Synonyms: Receptor-interacting serine/threonine-protein kinase 3, RIPK3,RIP3)
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Based on 1 Customer Validation

RIP3 Antibody (YA6043) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RIP3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, ELISA

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RIP3 Antibody (YA6043)
  • RIP3 Antibody (YA6043) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

RIP3 Antibody (YA6043) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RIP3.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719127

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RIP3 Antibody (YA6043)
    Western blot analysis of extracts from C2C12 (lane2(20μg), Mouse lung tissue (lane3(20μg), Mouse colon tissue (lane4(20μg) and RAW264.7 (lane5(20μg) using RIP3 Antibody (HY-P86351). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    RIP3 is a Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. In some cell types, also able to restrict viral replication by promoting cell death-independent responses. In response to Zika virus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate. Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production; (Microbial infection) In case of herpes simplex virus 1/HHV-1 infection, forms heteromeric amyloid structures with HHV-1 protein RIR1/ICP6 which may inhibit RIPK3-mediated necroptosis, thereby preventing host cell death pathway and allowing viral evasion[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus

  • Expression


    Tissue_specificity:Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney; Expression is significantly increased in colon and lung cancers

  • Isoforms & Post-Translational Modification

    Q9Y572 has 3 isomers: Q9Y572-1: 56887 Da (predicted); Q9Y572-2: 27574 Da (predicted); Q9Y572-3: 25326 Da (predicted).
    (Microbial infection) Proteolytically cleaved by S.flexneri OspD3 within the RIP homotypic interaction motif (RHIM), leading to its degradation and inhibition of necroptosis;RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (PubMed:19524513). Autophosphorylated following interaction with ZBP1 (By similarity). Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3 (PubMed:11734559, PubMed:19524512). Autophosphorylates at Ser-227 following activation by ZBP1: phosphorylation at these sites is a hallmark of necroptosis and is required for binding MLKL (PubMed:22265413). Phosphorylation at Thr-182 is important for its kinase activity, interaction with PELI1 and PELI1-mediated 'Lys-48'-linked polyubiquitination and for its ability to mediate TNF-induced necroptosis (PubMed:29883609);Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B (PubMed:21931591). Polyubiquitinated with 'Lys-48'-linked chains by PELI1 leading to its subsequent proteasome-dependent degradation. Ubiquitinated by STUB1 leading to its subsequent proteasome-dependent degradation (PubMed:29883609). Deubiquitinated by USP22 (PubMed:33369872)

  • Subunit

    Interacts (via RIP homotypic interaction motif) with RIPK1 (via RIP homotypic interaction motif); this interaction induces RIPK1 phosphorylation and formation of a RIPK1-RIPK3 necrosis-inducing complex (PubMed:10339433, PubMed:11734559, PubMed:19524512, PubMed:29681455).

  • SwissProt ID

    Q9Y572

  • Gene ID
    11035 [NCBI]
  • Synonyms

    Receptor-interacting serine/threonine-protein kinase 3, RIPK3,RIP3

RIP3 Antibody (YA6043) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C2C12 (lane2(20μg), Mouse lung tissue (lane3(20μg), Mouse colon tissue (lane4(20μg) and RAW264.7 (lane5(20μg) using RIP3 Antibody (HY-P86351). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • RIP3 Antibody (YA6043) Product Image
    1/16
  • Experimental Validation Results for RIP3 Antibody (YA6043)
  • RIP3 Antibody (YA6043) Product Image
  • Experimental Validation Results for RIP3 Antibody (YA6043)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C2C12 (lane2(20μg), Mouse lung tissue (lane3(20μg), Mouse colon tissue (lane4(20μg) and RAW264.7 (lane5(20μg) using RIP3 Antibody (HY-P86351). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RIP3 Antibody (YA6043)
  • RIP3 Antibody (YA6043) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C2C12 (lane2(20μg), Mouse lung tissue (lane3(20μg), Mouse colon tissue (lane4(20μg) and RAW264.7 (lane5(20μg) using RIP3 Antibody (HY-P86351). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • RIP3 Antibody (YA6043) Product Image WB