GTF2H1 Antibody (YA5206)

Cat. No.: HY-P85514: Data Sheet User Guide for Antibodies
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GTF2H1 Antibody (YA5206) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GTF2H1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GTF2H1 Antibody (YA5206) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GTF2H1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 62 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P32780

Gene ID

2965 [NCBI]

Immunogen

Purified recombinant human GTF2H1protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:300
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：P32780 has 2 isomers: P32780-1: 62032 Da (predicted); P32780-2: 48738 Da (predicted).
(Microbial infection) Upon Rift valley fever virus infection, interacts with NSs leading to ubiquitination by the NSs-FBXO3 E3 ligase and proteasome-dependent degradation

Subunit：Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60

Synonyms

Basic transcription factor 2 62 kDa subunit; Basic transcription factor 62 kDa subunit; BTF 2; BTF2; BTF2 p62; General transcription factor IIH polypeptide 1; General transcription factor IIH subunit 1; General transcription factor IIH, polypeptide 1, 62kDa; gtf2h1; TF2H1; TF2H1_HUMAN; TFB1; TFIIH; TFIIH basal transcription factor complex p62 subunit.

Documentation

User Guide for Antibodies (1077 KB)