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EpCAM Antibody (YA7610)

Customer Review

Based on 1 Customer Validation

EpCAM Antibody (YA7610) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to EpCAM.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • EpCAM Antibody (YA7610) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

EpCAM Antibody (YA7610) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to EpCAM.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EpCAM Antibody (YA7610)
    Western blot analysis of extracts from HCT116(lane 2(20ug) , MCF-7(lane 3(20ug) and HT-29(lane 4(20ug),A431(lane 5(20ug) using EpCAM Antibody (HY-P87925) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EpCAM Antibody (YA7610)
    Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EpCAM Antibody (YA7610)
    Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    EpCAM (CD326) is a transmembrane glycoprotein expressed on the surface of epithelial cells and serves as a classic marker for epithelial cells and epithelial-derived tumors. It plays a role in cell adhesion, the regulation of proliferation, and the maintenance of tumor stemness, holding significant value in tumor diagnosis, circulating tumor cell detection, cancer stem cell research, and the development of targeted therapies.

  • Subcellular Localization

    Lateral cell membrane,Cell junction, tight junction

  • Expression


    Tissue_specificity:This gene is highly and selectively expressed in undifferentiated embryonic stem cells (ESCs) , but not in differentiated ESCs. Once ESCs differentiate, its protein level rapidly declines. This gene is expressed on almost all epithelial cell membranes, but not on mesoderm or neural cell membranes. It has also been found on the surface of adenocarcinomas.

  • Subunit

    Monomer. Interacts with phosphorylated CLDN7

  • SwissProt ID

    P16422

EpCAM Antibody (YA7610) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20ug) , MCF-7(lane 3(20ug) and HT-29(lane 4(20ug),A431(lane 5(20ug) using EpCAM Antibody (HY-P87925) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EpCAM Antibody (YA7610) Product Image
    1/16
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • EpCAM Antibody (YA7610) Product Image
  • Experimental Validation Results for EpCAM Antibody (YA7610)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 2(20ug) , MCF-7(lane 3(20ug) and HT-29(lane 4(20ug),A431(lane 5(20ug) using EpCAM Antibody (HY-P87925) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EpCAM Antibody (YA7610)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EpCAM Antibody (YA7610)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • Experimental Validation Results for EpCAM Antibody (YA7610)
  • EpCAM Antibody (YA7610) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116(lane 2(20ug) , MCF-7(lane 3(20ug) and HT-29(lane 4(20ug),A431(lane 5(20ug) using EpCAM Antibody (HY-P87925) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • EpCAM Antibody (YA7610) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EpCAM Antibody (YA7610) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling EpCAM with EpCAM Antibody (HY-P87925) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EpCAM Antibody (HY-P87925) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EpCAM Antibody (YA7610) Product Image WB
  • EpCAM Antibody (YA7610) Product Image ICC
  • EpCAM Antibody (YA7610) Product Image ICC