Beclin 1 Antibody (YA587)(PBS only)

Art. -Nr.: HY-P80568A: Data Sheet User Guide for Antibodies
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Beclin 1 Antibody (YA587) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe		Verfügbarkeit
50 μL		Angebot einholen
100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

Beclin 1 Antibody (YA587) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

Host

Rabbit

Molekulargewicht

Predicted band size: 52 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q14457

Gene ID

8678 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Beclin 1.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Reinheit	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors; (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus

Subcellular Localization：Cytoplasm; Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Peripheral membrane protein; Mitochondrion membrane; Peripheral membrane protein; Endosome; Cytoplasmic vesicle, autophagosome; Mitochondrion; Nucleus; Cytoplasm; Mitochondrion

Expression：
Tissue_specificity:general expression

Subunit：A homodimeric form is proposed to exist; this metastable form readily transits to ATG14- or UVRAG-containing complexes with BECN1:UVRAG being more stable than BECN1:ATG14 (By similarity).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

BECN1

Dokumentation

User Guide for Antibodies (1077 KB)