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Beclin 1 Antibody (YA587)(PBS only)

(Synonyms: BECN1)

Beclin 1 Antibody (YA587) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
  • Beclin 1 Antibody (YA587)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Beclin 1 Antibody (YA587) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Immunogen

Synthetic peptide corresponding to Human Beclin 1.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
    Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) and HEK293(lane 4(20ug) using Beclin 1 Antibody (HY-P80568) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
    Western blot analysis of extracts from K562 (lane 2(40μg), C6 (lane 3(40μg), 3T3 (lane 4(40μg)using Beclin 1 Antibody (YA587). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Beclin 1, also named Atg6, is highly conserved in eukaryotes and belongs to theautophagy-related (Atg) family of proteins. Beclin 1 is a well-established regulator of autophagy, a process essential for mammalian survival. Beclin 1 performs both of its autophagy and membrane trafficking functions by interacting with several other proteins—primarily vacuolar protein sorting-associated protein 15 (VPS15), VPS34, UV radiation resistance-associated gene product (UVRAG), and autophagy-related protein 14 (ATG14) . Together, these assemble into two different Class III PI3K complexes, Complex 1 (C1) and Complex 2 (C2), depending on whether ATG14 or UVRAG respectively is present. Within these complexes, the catalytic lipid kinase subunit, VSP34, is responsible for the phosphorylation of PtdIns, which then mediates autophagy and/or membrane trafficking functions through the recruitment of effector proteins.

  • Subcellular Localization

    Cytoplasm; Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Peripheral membrane protein; Mitochondrion membrane; Peripheral membrane protein; Endosome; Cytoplasmic vesicle, autophagosome; Mitochondrion; Nucleus; Cytoplasm; Mitochondrion

  • Expression


    Tissue_specificity:general expression

  • Subunit

    A homodimeric form is proposed to exist; this metastable form readily transits to ATG14- or UVRAG-containing complexes with BECN1:UVRAG being more stable than BECN1:ATG14 (By similarity).

  • SwissProt ID

    Q14457

  • Gene ID
    8678 [NCBI]
  • Synonyms

    BECN1

  • Research Field

    Epigenetics and Nuclear Signaling

Beclin 1 Antibody (YA587)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) and HEK293(lane 4(20ug) using Beclin 1 Antibody (HY-P80568) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(40μg), C6 (lane 3(40μg), 3T3 (lane 4(40μg)using Beclin 1 Antibody (YA587). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Beclin 1 Antibody (YA587)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
  • Beclin 1 Antibody (YA587)(PBS only) Product Image
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) and HEK293(lane 4(20ug) using Beclin 1 Antibody (HY-P80568) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(40μg), C6 (lane 3(40μg), 3T3 (lane 4(40μg)using Beclin 1 Antibody (YA587). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
  • Experimental Validation Results for Beclin 1 Antibody (YA587)(PBS only)
  • Beclin 1 Antibody (YA587)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg) , HepG2(lane 3(20μg) and HEK293(lane 4(20ug) using Beclin 1 Antibody (HY-P80568) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Beclin 1 Antibody (YA587)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(40μg), C6 (lane 3(40μg), 3T3 (lane 4(40μg)using Beclin 1 Antibody (YA587). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Beclin 1 Antibody (YA587)(PBS only) Product Image WB
  • Beclin 1 Antibody (YA587)(PBS only) Product Image WB