2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Fas Antibody (YA446)

Fas Antibody (YA446) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fas.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

  • References

Description

Fas Antibody (YA446) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fas.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 38 kDa;
Observed band size: 40-50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human Fas.AA range:130-335.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro)
Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Secreted; Secreted; Secreted; Secreted; Secreted
Expression：
Tissue_specificity:Subtype 1 and subtype 6 were expressed at equal levels in resting peripheral blood mononuclear cells. Upon activation, subtype 1 levels increased, while subtype 6 levels decreased.
Isoforms & Post-Translational Modification：P25445 has 7 isomers: P25445-1: 37732 Da (predicted); P25445-2: 11435 Da (predicted); P25445-3: 9390 Da (predicted); P25445-4: 16648 Da (predicted); P25445-5: 14602 Da (predicted); P25445-6: 35386 Da (predicted); P25445-7: 24781 Da (predicted).
(Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions;Palmitoylated (PubMed:25301068). Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane (PubMed:25301068);N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans
Subunit：Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits (PubMed:21109225, PubMed:9184224, PubMed:9322534).
Database

Entrez Gene: 355 Human

SwissProt: P25445 Human

OMIM: 601859 Human

Research Field

Cell Biology
Documentation
User Guide for Antibodies (1077 KB)
References

Fas Antibody (YA446) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Fas Antibody (YA446)
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