ELMO1 Antibody (YA2243)

Cat. No.: HY-P82498: Ficha de datos COA
SDS
User Guide for Antibodies
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ELMO1 Antibody (YA2243) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELMO1.

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Tamaño	Stock	Cantidad
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
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ELMO1 Antibody (YA2243) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

ELMO1 Antibody (YA2243) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELMO1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Peso molecular

Predicted band size: 84 kDa;

Observed band size: 84 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q92556

Gene ID

9844 [NCBI]

Immunogen

A synthesized peptide derived from human ELMO1 aa680-727/727.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Pureza	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from Jurkat (lane 2(20μg), C6 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using ELMO1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling ELMO1 with ELMO1 Antibody (HY-P82498) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELMO1 Antibody (HY-P82498) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of K562 cells labeling ELMO1 with ELMO1 Antibody (HY-P82498) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ELMO1 Antibody (HY-P82498)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1

Subcellular Localization：Cytoplasm; Cell membrane

Expression：
Tissue_specificity:Widely expressed, with a higher expression in the spleen and placenta

Isoforms & Post-Translational Modification：Q92556 has 3 isomers: Q92556-1: 83829 Da (predicted); Q92556-2: 28742 Da (predicted); Q92556-3: 49915 Da (predicted).
Phosphorylated by HCK

Subunit：Interacts with ADGRB1 (By similarity). Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. Part of a complex with DOCK1 and RAC1. Part of a complex with DOCK1 and CRK isoform CRK-II. Interacts with PLEKHG6. Interacts with HCK (via SH3 domain). Interacts with ADGRB3 (PubMed:24567399). Interacts with DOCK5 (By similarity)

RRID

AB_3104605

Database

Entrez Gene: 9844 Human ; 140580 Mouse ; 361251 Rat

SwissProt: Q92556 Human ; Q8BPU7 Mouse ;

OMIM: 606420 Human

Research Field

Signal Transduction

Synonyms

ELMO1; KIAA0281; Engulfment and cell motility protein 1; Protein ced-12 homolog

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

ELMO1 Antibody (YA2243) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ELMO1 Antibody (YA2243)

ELMO1 Antibody (YA2243) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

ELMO1 Antibody (YA2243) Related Classifications

Productos vistos recientemente:

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