Copy Link Print Information

VPS41 Antibody (YA2409)

(Synonyms: HVPS41; HVSP41; hVps41p)
Customer Review

Based on 1 Customer Validation

VPS41 Antibody (YA2409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VPS41.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VPS41 Antibody (YA2409)
  • VPS41 Antibody (YA2409) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

VPS41 Antibody (YA2409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VPS41.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 99 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 99 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human VPS41

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VPS41 Antibody (YA2409)
    Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2), Rat brain (lane 3), 293T (lane 4), HeLa (lane 5), Mouse brain (lane 6), RAW264.7 (lane 7), and K562 (lane 8) using VPS41 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    VPS41 plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Acts as a component of the HOPS endosomal tethering complex. This complex is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes implicated in degradation of endocytosed cargo. Required for fusion of autophagosomes with lysosomes. Links the HOPS complex to endosomal Rab7 via its association with RILP and to lysosomal membranes via its association with ARL8B, suggesting that these interactions may bring the compartments to close proximity for fusion. Involved in the direct trans-Golgi network to late endosomes transport of lysosomal membrane proteins independently of HOPS. Involved in sorting to the regulated secretory pathway presumably implicating the AP-3 adapter complex. May play a role in HOPS-independent function in the regulated secretory pathway[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Endosome membrane; Peripheral membrane protein; Late endosome membrane; Peripheral membrane protein; Early endosome membrane; Peripheral membrane protein; Lysosome membrane; Peripheral membrane protein; Golgi apparatus, trans-Golgi network; Cytoplasmic vesicle, clathrin-coated vesicle; Cytoplasm, cytosol

  • Expression


    Tissue_specificity:Expressed in cerebral cortex and cerebellum. Highly expressed in Purkinje cells

  • Isoforms & Post-Translational Modification

    P49754 has 3 isomers: P49754-1: 98566 Da (predicted); P49754-2: 92846 Da (predicted); P49754-3: 95924 Da (predicted).

  • Subunit

    Component of the homotypic fusion and vacuole protein sorting (HOPS) complex; the core of which composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, is associated with VPS39 and VPS41 (PubMed:23351085, PubMed:25445562, PubMed:25908847, PubMed:33851776). Interacts with RILP, MON1B (PubMed:20434987, PubMed:25445562). Interacts with ARL8B (GTP-bound form); involved in recruitment to lysosomes and probably hierarchial assembly of the HOPS complex at lysosomal membranes (PubMed:25908847). In vitro can self-assemble into a lattice (PubMed:24210660). Associates with adapter protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634). Interacts with STX17; this interaction is increased in the absence of TMEM39A (PubMed:31806350, PubMed:33422265). Interacts with ARL8B and PLEKHM1; the interaction mediates the recruitment of the HOPS complex to lysosomes (PubMed:21802320, PubMed:25908847, PubMed:28325809). Interacts with RAB7, RAB2A and RAB2B (By similarity). Interacts with RAB39A (GTP-bound) and RAB39B (GTP-bound); interaction with RAB39A leads to a functional HOPS complex that mediates autophagosome-lysosome membrane tethering (PubMed:37821429)

  • SwissProt ID

    P49754

  • Gene ID
    27072 [NCBI]
  • Synonyms

    HVPS41; HVSP41; hVps41p

  • Research Field

    Signal Transduction

References

[1]. Solinger JA, et al. Tethering complexes in the endocytic pathway: CORVET and HOPS. FEBS J. 2013 Jun;280(12):2743-57. [Content Brief]

[2]. van der Welle REN, et al. Neurodegenerative VPS41 variants inhibit HOPS function and mTORC1-dependent TFEB/TFE3 regulation. EMBO Mol Med. 2021 May 7;13(5):e13258. [Content Brief]

[3]. Pols MS, et al. The HOPS proteins hVps41 and hVps39 are required for homotypic and heterotypic late endosome fusion. Traffic. 2013 Feb;14(2):219-32. [Content Brief]

[4]. Lin X, et al. RILP interacts with HOPS complex via VPS41 subunit to regulate endocytic trafficking. Sci Rep. 2014 Dec 2;4:7282. [Content Brief]

[5]. Khatter D, et al. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes. J Cell Sci. 2015 May 1;128(9):1746-61. [Content Brief]

[6]. Radisky DC, et al. Characterization of VPS41, a gene required for vacuolar trafficking and high-affinity iron transport in yeast. Proc Natl Acad Sci U S A. 1997 May 27;94(11):5662-6. [Content Brief]

[7]. Wartosch L, et al. Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes. Traffic. 2015 Jul;16(7):727-42. [Content Brief]

[8]. Zhang S, et al. C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy. Nat Commun. 2023 Oct 11;14(1):6360. [Content Brief]

[9]. Garg S, et al. Lysosomal trafficking, antigen presentation, and microbial killing are controlled by the Arf-like GTPase Arl8b. Immunity. 2011 Aug 26;35(2):182-93. [Content Brief]

[10]. Pols MS, et al. hVps41 and VAMP7 function in direct TGN to late endosome transport of lysosomal membrane proteins. Nat Commun. 2013;4:1361. [Content Brief]

[11]. Asensio CS, et al. Self-assembly of VPS41 promotes sorting required for biogenesis of the regulated secretory pathway. Dev Cell. 2013 Nov 25;27(4):425-37. [Content Brief]

View More

VPS41 Antibody (YA2409) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2), Rat brain (lane 3), 293T (lane 4), HeLa (lane 5), Mouse brain (lane 6), RAW264.7 (lane 7), and K562 (lane 8) using VPS41 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • VPS41 Antibody (YA2409) Product Image
    1/16
  • Experimental Validation Results for VPS41 Antibody (YA2409)
  • VPS41 Antibody (YA2409) Product Image
  • Experimental Validation Results for VPS41 Antibody (YA2409)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2), Rat brain (lane 3), 293T (lane 4), HeLa (lane 5), Mouse brain (lane 6), RAW264.7 (lane 7), and K562 (lane 8) using VPS41 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for VPS41 Antibody (YA2409)
  • VPS41 Antibody (YA2409) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Rat heart (lane 2), Rat brain (lane 3), 293T (lane 4), HeLa (lane 5), Mouse brain (lane 6), RAW264.7 (lane 7), and K562 (lane 8) using VPS41 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • VPS41 Antibody (YA2409) Product Image WB