Dynamin 2 Antibody (YA2464)

Cat. No.: HY-P82719: Data Sheet COA
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Dynamin 2 Antibody (YA2464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dynamin 2.

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
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Dynamin 2 Antibody (YA2464) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation
References

Description

Dynamin 2 Antibody (YA2464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dynamin 2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 98 kDa;

Observed band size: 98 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P50570

Gene ID

1785 [NCBI]

Immunogen

A synthesized peptide derived from human Dynamin 2 aa750-800/870.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562 (lane 2, 20 μg),Jurkat (lane 3, 20 μg), Raji (lane 4, 20 μg) and Rat brain (lane 5, 20 μg) using Dynamin 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission at plasma membrane during endocytosis and filament remodeling at many actin structures during organization of the actin cytoskeleton (PubMed:15731758, PubMed:19605363, PubMed:19623537, PubMed:33713620, PubMed:34744632). Plays an important role in vesicular trafficking processes, namely clathrin-mediated endocytosis (CME), exocytic and clathrin-coated vesicle from the trans-Golgi network, and PDGF stimulated macropinocytosis (PubMed:15731758, PubMed:19623537, PubMed:33713620). During vesicular trafficking process, associates to the membrane, through lipid binding, and self-assembles into ring-like structure through oligomerization to form a helical polymer around the vesicle membrane and leading to vesicle scission (PubMed:17636067, PubMed:34744632, PubMed:36445308). Plays a role in organization of the actin cytoskeleton by mediating arrangement of stress fibers and actin bundles in podocytes (By similarity). During organization of the actin cytoskeleton, self-assembles into ring-like structure that directly bundles actin filaments to form typical membrane tubules decorated with dynamin spiral polymers (By similarity). Self-assembly increases GTPase activity and the GTP hydrolysis causes the rapid depolymerization of dynamin spiral polymers, and results in dispersion of actin bundles (By similarity). Remodels, through its interaction with CTTN, bundled actin filaments in a GTPase-dependent manner and plays a role in orchestrating the global actomyosin cytoskeleton (PubMed:19605363). The interaction with CTTN stabilizes the interaction of DNM2 and actin filaments and stimulates the intrinsic GTPase activity that results in actin filament-barbed ends and increases the sensitivity of filaments in bundles to the actin depolymerizing factor, CFL1 (By similarity). Plays a role in the autophagy process, by participating in the formation of ATG9A vesicles destined for the autophagosomes through its interaction with SNX18 (PubMed:29437695), by mediating recycling endosome scission leading to autophagosome release through MAP1LC3B interaction (PubMed:29437695, PubMed:32315611). Also regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane (By similarity). Also plays a role in cytokinesis (By similarity). May participate in centrosome cohesion through its interaction with TUBG1 (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Involved in membrane tubulation (PubMed:24135484)

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasmic vesicle, clathrin-coated vesicle; Cell projection, uropodium; Endosome; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Recycling endosome; Cell projection, phagocytic cup; Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein; Cell projection, podosome; Cytoplasm; Cell junction; Postsynaptic density; Synapse, synaptosome; Midbody; Membrane, clathrin-coated pit

Expression：
Tissue_specificity:Widely expressed (PubMed: 7590285) . Expressed in skeletal muscle and peripheral nerves (PubMed: 19623537) .

Isoforms & Post-Translational Modification：P50570 has 5 isomers: P50570-1: 98064 Da (predicted); P50570-2: 97652 Da (predicted); P50570-3: 97554 Da (predicted); P50570-4: 97966 Da (predicted); P50570-5: 97977 Da (predicted).
Phosphorylation at Ser-848 by GSK3-alpha relieves the inhibition of BIN1 and promotes endocytosis (PubMed:36445308). Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry (PubMed:20496096). It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis (By similarity). Dephosphorylated by calcineurin/PP2 during cytokinesis in a Ca(2+)- and calmodulin-dependent manner (PubMed:20496096). Phosphorylated on tyrosine residues by EGFR and after activation of SRC (By similarity)

Subunit：Oligomerizes into a helical polymer that self-assembles around the vesicle membrane, when associated to the menbrane through lipid binding (PubMed:34744632, PubMed:36445308). Interacts with SHANK1 and SHANK2 (PubMed:11583995). Interacts with SNX9 (PubMed:15703209). Interacts (via C-terminal proline-rich domain (PRD)) with SNX18 (via SH3 domain); this interaction regulates ATG9A and ATG16L1 trafficking from recycling endosomes to sites of autophagosome formation (PubMed:29437695, PubMed:32315611). Interacts with SNX33 (via SH3 domain) (PubMed:18353773). Interacts with MYO1E (via SH3 domain) (PubMed:17257598). Interacts with PSTPIP1 (via SH3 domain) (PubMed:18480402). Interacts with CTNND2 (PubMed:22022388). Interacts (via C-terminal proline-rich domain (PRD)) with BIN1 (via SH3 domain); this interaction allows the recruitment of DNM2 to the membrane tubules and inhibits self-assembly-stimulated GTPase activity on the membrane (PubMed:17676042, PubMed:36445308). Interacts with GABARAP, GABARAPL1 and GABARAPL2 (PubMed:32315611). Interacts with MAP1LC3B (the lipidate and non-lipidated LC3 form); this interaction mediates recycling endosome scission leading to autophagosome release (PubMed:32315611). Interacts with ITSN1 (PubMed:32315611). Interacts (via C-terminal proline-rich domain (PRD)) with SH3BP4 (via SH3 domain); this interaction controls the GTPase activity and is prevented by EGFR-induced tyrosine phosphorylation of either DNM2 or SH3BP4 (PubMed:16325581). May interact with PIK3C3. May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3. Interacts with SDC4; this interaction is markedly enhanced at focal ahesion site upon induction of focal adhesions and stress-fiber formation (By similarity). Interacts with ACTN1. Interacts with CTTN; this interaction stimulates the intrinsic GTPase activity of DNM2 and stabilizes the association of DNM2 and actin filaments; in addition this interaction is stimulated by ligand binding to the receptor, leading to the recruitment of the DNM2-CTTN complex to the sequestered receptor-ligand complex to its internalization. Interacts with NOSTRIN (via SH3 domain); this interaction allows the recruitment of NOS3 to dynamin-positive structures. Interacts with TUBG1; this interaction may participate in centrosome cohesion (By similarity)

RRID

AB_3104818

Database

Entrez Gene: 1785 Human ; 13430 Mouse ; 25751 Rat

SwissProt: P50570 Human ; P39054 Mouse ; P39052 Rat

OMIM: 160150 Human

Research Field

Signal Transduction

Synonyms

DNM2; DYN2; Dynamin-2

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/ht-1080-cell.html [Content Brief]

Dynamin 2 Antibody (YA2464) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Dynamin 2 Antibody (YA2464)

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Background

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Dynamin 2 Antibody (YA2464) Related Classifications

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