PIWIL1 Antibody (YA9733)
(Synonyms: HIWI, MIWI, Piwi (Drosophila) like 1, PIWI, Piwi homolog, Piwi like 1 (drosophila), Piwi like 1, Piwi-like protein 1, PIWIL1, PIWL1_HUMAN)PIWIL1 Antibody (YA9733) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to PIWIL1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-200 |
Product Details
PIWIL1 Antibody (YA9733) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to PIWIL1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 99 kDa;
Recombinant protein of Human PIWIL1 (aa 733-831).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PIWIL1 is an Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1
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Subcellular Localization
Cytoplasm
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Expression
Tissue_Specificity: Expressed in spermatocytes and spermatids. Also detected in prostate cancer (at protein level). Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3: Ubiquitously expressed, and specifically in CD34(+) hematopoietic progenitor cells but not in more differentiated cells.
Induction: Down-regulated in CD34(+) hematopoietic cells during differentiation. -
Isoforms & Post-Translational Modification
PIWIL1 has 3 isoforms, Q96J94-1: amino acid length is 861, molecular weight is 98603 Da (predicted); Q96J94-2: amino acid length is 829, molecular weight is 94845 Da (predicted); Q96J94-3: amino acid length is 775, molecular weight is 89484 Da (predicted).
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. -
Subunit
Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes; this interaction is regulated by HSP90AB1 activity.
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SwissProt ID
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Synonyms
HIWI, MIWI, Piwi (Drosophila) like 1, PIWI, Piwi homolog, Piwi like 1 (drosophila), Piwi like 1, Piwi-like protein 1, PIWIL1, PIWL1_HUMAN
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