Copy Link Print Information

Hes1 Antibody (YA387)

(Synonyms: bHLHb39 antibody; C-HAIRY1 antibody; Class B basic helix-loop-helix protein 39 antibody; FLJ20408 antibody; Hairy and enhancer of split 1 antibody; Hairy homolog (Drosophila) antibody; Hairy homolog antibody; Hairy like antibody; Hairy, Drosophila, homolog of antibody; Hairy-like protein antibody; HAIRY1 antibody; HES-1 antibody; HES1_HUMAN antibody; HHL antibody; HL antibody; HRY antibody; MGC129109 antibody; OTTHUMP00000209031 antibody; RHL antibody; Transcription factor HES-1 antibody)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Hes1 Antibody (YA387) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hes1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Hes1 Antibody (YA387)
  • Experimental Validation Results for Hes1 Antibody (YA387)
  • Hes1 Antibody (YA387) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Hes1 Antibody (YA387)

More

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:100-1:500 1:50-1:400 1:50-1:100

Product Details

Description

Hes1 Antibody (YA387) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hes1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 30 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Hes1.AA range:298-241.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102321

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Hes1 Antibody (YA387)
    Western blot analysis of extracts from MDA-MB-231 (lane 1) and 293T (lane 2) using Hes1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Hes1 Antibody (YA387)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Hes1 Antibody(HY-P80155, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Hes1 transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage

  • Subcellular Localization

    Nucleus

  • Subunit

    Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.

  • SwissProt ID

    Q14469

  • Gene ID
    3280 [NCBI]
  • Synonyms

    bHLHb39 antibody; C-HAIRY1 antibody; Class B basic helix-loop-helix protein 39 antibody; FLJ20408 antibody; Hairy and enhancer of split 1 antibody; Hairy homolog (Drosophila) antibody; Hairy homolog antibody; Hairy like antibody; Hairy, Drosophila, homolog of antibody; Hairy-like protein antibody; HAIRY1 antibody; HES-1 antibody; HES1_HUMAN antibody; HHL antibody; HL antibody; HRY antibody; MGC129109 antibody; OTTHUMP00000209031 antibody; RHL antibody; Transcription factor HES-1 antibody

  • Research Field

    Neuroscience

Hes1 Antibody (YA387) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB-231 (lane 1) and 293T (lane 2) using Hes1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Hes1 Antibody(HY-P80155, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Hes1 Antibody (YA387) Product Image
    1/16
  • Experimental Validation Results for Hes1 Antibody (YA387)
  • Experimental Validation Results for Hes1 Antibody (YA387)
  • Hes1 Antibody (YA387) Product Image
  • Experimental Validation Results for Hes1 Antibody (YA387)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB-231 (lane 1) and 293T (lane 2) using Hes1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Hes1 Antibody (YA387)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Hes1 Antibody(HY-P80155, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Hes1 Antibody (YA387)
  • Experimental Validation Results for Hes1 Antibody (YA387)
  • Hes1 Antibody (YA387) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MDA-MB-231 (lane 1) and 293T (lane 2) using Hes1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Hes1 Antibody (YA387) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Hes1 Antibody(HY-P80155, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Hes1 Antibody (YA387) Product Image WB
  • Hes1 Antibody (YA387) Product Image FC