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Phospho-AKT1 (Ser473) Antibody (YA228)

(Synonyms: AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha)
4 Cited Publications
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Based on 4 publication(s) in Google Scholar

Phospho-AKT1 (Ser473) Antibody (YA228) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-AKT1 (Ser473).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    1.Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide.
    2.Supplied in PBS (pH 7.4), 150 mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
  • Phospho-AKT1 (Ser473) Antibody (YA228) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:2000-1:5000 1:100-1:200 1:50

Product Details

Description

Phospho-AKT1 (Ser473) Antibody (YA228) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-AKT1 (Ser473).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 56 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 56 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser473 of Human AKT1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102190

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide.
    2.Supplied in PBS (pH 7.4), 150 mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
    Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using Phospho-AKT1 (Ser473) (HY-P80788) Rabbit mAb. Pro-teins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
    Western blot analysis of extracts from C6 (lane 2(40μg), 3T3 (lane 3(40μg), Hela (lane 4(40μg) using Phospho-AKT1 (Ser473) Antibody (YA228). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    AKT1 (protein kinase Bα, PKBα) is a serine/threonine kinase that functions as a central effector of the PI3K/AKT/mTOR signaling pathway, regulating cellular growth, survival, metabolism, proliferation, and differentiation across multiple tissues and cell types[1][2][3]. Activation of AKT1 occurs downstream of PI3K through phosphoinositide-dependent kinase-1 (PDK1) -mediated phosphorylation, enabling downstream regulation of targets involved in protein synthesis, apoptosis control, and metabolic adaptation[4][5]. Mechanistically, AKT1 contributes to angiogenesis, physiological tissue growth, immune-cell function, and stress-response signaling, making it a critical node in both normal physiology and disease-associated signaling networks[2][4][5]. In disease models, dysregulated AKT signaling is strongly associated with cancer, metabolic disorders, cardiovascular pathology, and inflammatory conditions, and constitutive activation of AKT-dependent pathways promotes oncogenic progression and therapeutic resistance[2][6][7]. Compared with related isoforms, AKT1 exhibits distinct biological functions despite substantial structural homology with AKT2 and AKT3; genetic studies demonstrate that AKT1 deficiency primarily causes growth retardation, whereas AKT2 deficiency induces insulin resistance and diabetes, and AKT3 loss predominantly affects brain development[4][5]. Isoform-specific investigations further show that AKT1 and AKT2 can exert non-redundant or even opposing effects on migration, invasion, inflammation, and fibrogenesis depending on cellular context[2][6][8]. For experimental applications, increasing efforts focus on developing AKT1-selective inhibitors and isoform-selective cellular models, which provide valuable tools for dissecting AKT isoform-specific signaling and for evaluating targeted therapeutic strategies with improved selectivity profiles[6][7][9].

  • Subcellular Localization

    Cytoplasm; Nucleus; Cell membrane; Mitochondrion intermembrane space

  • Expression


    Tissue_specificity:This protein is expressed in prostate cancer, and its expression level gradually increases from normal to malignant (protein level) . It is expressed in all human cell types analyzed to date. The phosphorylated form of Tyr-176 is significantly increased in the progressive stages of breast cancer (i.e., from normal to hyperplastic (ADH) , ductal carcinoma in situ (DCIS) , invasive ductal carcinoma (IDC) , and lymph node metastasis (LNMM) stages) .

  • Isoforms & Post-Translational Modification

    AKT1 has 2 isomers: P31749-1: 55686 Da (predicted); P31749-2: 48347 Da (predicted).
    O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site;Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Phosphorylation of the activation loop at Thr-308 by PDPK1/PDK1 is a prerequisite for full activation. Phosphorylation by mTORC2 in response to growth factors plays a key role in AKT1 activation: mTORC2 phosphorylates different sites depending on the context, such as Thr-450, Ser-473, Ser-477 or Thr-479, thereby facilitating subsequent phosphorylation of the activation loop by PDPK1/PDK1.

  • Subunit

    Interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A and TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.

  • SwissProt ID

    P31749

  • Gene ID
    207 [NCBI]
  • Synonyms

    AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

  • Research Field

    Neuroscience

[1]. Guerau-de-Arellano M, et al. Akt isoforms in the immune system. Front Immunol. 2022 Aug 23;13:990874. [Content Brief]

[2]. Reyes-Gordillo K, et al. Akt1 and Akt2 Isoforms Play Distinct Roles in Regulating the Development of Inflammation and Fibrosis Associated with Alcoholic Liver Disease. Cells. 2019 Oct 29;8(11):1337. [Content Brief]

[3]. O'Neill BT, et al. Akt1 in the cardiovascular system: friend or foe? J Clin Invest. 2005 Aug;115(8):2059-64. [Content Brief]

[4]. Tellai AD, et al. The complex post-transcriptional regulation of genes coding for methionine adenosyl transferase: New insights for liver cancer. Biochimie. 2025 Nov;238(Pt A):19-28. [Content Brief]

[5]. Quambusch L, et al. Cellular model system to dissect the isoform-selectivity of Akt inhibitors. Nat Commun. 2021 Sep 6;12(1):5297. [Content Brief]

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Phospho-AKT1 (Ser473) Antibody (YA228) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using Phospho-AKT1 (Ser473) (HY-P80788) Rabbit mAb. Pro-teins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(40μg), 3T3 (lane 3(40μg), Hela (lane 4(40μg) using Phospho-AKT1 (Ser473) Antibody (YA228). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-AKT1 (Ser473) Antibody (YA228) Product Image
    1/16
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
  • Phospho-AKT1 (Ser473) Antibody (YA228) Product Image
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using Phospho-AKT1 (Ser473) (HY-P80788) Rabbit mAb. Pro-teins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(40μg), 3T3 (lane 3(40μg), Hela (lane 4(40μg) using Phospho-AKT1 (Ser473) Antibody (YA228). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
  • Experimental Validation Results for Phospho-AKT1 (Ser473) Antibody (YA228)
  • Phospho-AKT1 (Ser473) Antibody (YA228) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using Phospho-AKT1 (Ser473) (HY-P80788) Rabbit mAb. Pro-teins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-AKT1 (Ser473) Antibody (YA228) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(40μg), 3T3 (lane 3(40μg), Hela (lane 4(40μg) using Phospho-AKT1 (Ser473) Antibody (YA228). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-AKT1 (Ser473) Antibody (YA228) Product Image WB
  • Phospho-AKT1 (Ser473) Antibody (YA228) Product Image WB