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  5. AKT1+AKT2+AKT3 Antibody (YA635)

AKT1+AKT2+AKT3 Antibody (YA635)

Cat. No.: HY-P80535
COA
SDS
User Guide for Antibodies Technical Support

AKT1+AKT2+AKT3 Antibody (YA635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AKT1+AKT2+AKT3.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

    AKT1+AKT2+AKT3 Antibody (YA635) purchased from MCE. Usage Cited in: Phytomedicine. 2025 Dec 26:150:157741.  [Abstract]

    A549 and LLC cells were treated with increasing doses of TXC for 24 h, followed by western blotting for ERBB2, PI3K, phospho-PI3K, AKT, phospho-AKT, mTOR, phospho-Mtor.

    AKT1+AKT2+AKT3 Antibody (YA635) purchased from MCE. Usage Cited in: Viruses. 2025 Dec 17;17(12):1637.  [Abstract]

    Quercetin suppresses the PI3K/Akt/mTOR signaling pathway. MARC-145 cells were infected with PRRSV (MOI of 0.8) and treated with quercetin for 48 h.
    • WB: Western Blot;
    • IHC-P: Immunohistochemistry-Paraffin;
    • IHC-F: Immunohistochemistry-Frozen;
    • ICC/IF: Immunocytochemistry/Immunofluorescence;
    • IF-Tissue: Immunofluorescence-Tissue;
    • mIHC: Multiplex Immunohistochemical;
    • IP: Immunoprecipitation;
    • ChIP: Chromatin Immunoprecipitation;
    • FC: Flow Cytometry;
    • ELISA: Enzyme Linked Immunosorbent Assay

    • Product Detail

    • Verification Image

    • Background

    • Documentation

    Description

    AKT1+AKT2+AKT3 Antibody (YA635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AKT1+AKT2+AKT3.
    Host

    Rabbit
    Clonality

    Recombinant, Monoclonal
    Molecular Weight
    Predicted band size: 56 kDa;
    Observed band size: 56 kDa
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
    Species Reactivity
    Human, Mouse, Rat, Hamster
    SwissProt ID
    Gene ID
    Immunogen

    Synthetic peptide corresponding to Human AKT1/2/3 aa:281-480.
    Application &
    Dilution Ratio
    Application Dilution Ratio
    WB
    WB: Western Blot
    		1:500-1:1000
    IHC-P
    IHC-P: Immunohistochemistry-Paraffin
    		1:50-1:100
    IHC-F
    IHC-F: Immunohistochemistry-Frozen
    		1:50-1:100
    ICC/IF
    ICC/IF: Immunocytochemistry/Immunofluorescence
    		1:50-1:200
    IP
    IP: Immunoprecipitation
    		1:20
    Sensitivity Endogenous Purity affinity purified
    Conjugation Non-conjugated Modification Unmodified
    Isotype IgG  
    Appearance

    Liquid
    Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
    Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
    Shipping

    Shipping with blue ice.
    Verification Image
    ALL WB IHC-P

    • Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and C6(lane 4(20μg) using AKT1/AKT2/AKT3 (HY-P80535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

    • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Background
    Function：P31749: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis (PubMed:11882383, PubMed:15526160, PubMed:15861136, PubMed:21432781, PubMed:21620960, PubMed:31204173). This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates (PubMed:11882383, PubMed:15526160, PubMed:21432781, PubMed:21620960, PubMed:29343641, PubMed:31204173).
    P31751: Serine/threonine kinase closely related to AKT1 and AKT3. All 3 enzymes, AKT1, AKT2 and AKT3, are collectively known as AKT kinase. AKT regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis, through the phosphorylation of a range of downstream substrates.
    Q9Y243: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates.
    Subcellular Localization：P31749: Cytoplasm; Nucleus; Cell membrane; Mitochondrion intermembrane space
    P31751: Cytoplasm; Nucleus; Cell membrane; Peripheral membrane protein; Early endosome
    Q9Y243: Nucleus; Cytoplasm; Membrane; Peripheral membrane protein
    Expression：
    Tissue_specificity: P31749: Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH) , ductal carcinoma in situ (DCIS) , invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages
    P31751: Widely expressed. Expressed in myoblasts (PubMed:17565718)
    Q9Y243: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney
    Subunit：P31749: Interacts with BTBD10 (By similarity). Interacts with KCTD20 (By similarity). Interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10.
    P31751: Interacts with BTBD10 (By similarity).
    Q9Y243: Interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.
    RRID
    Database

    Entrez Gene: 207 Human ; 208 Human ; 10000 Human

    SwissProt: P31749 Human ; P31751 Human ; Q9Y243 Human ;

    OMIM: 114480 Human

    Research Field

    Signal Transduction
    Synonyms
    AKT1,AKT2,AKT3
    Documentation
    SDS (251 KB)

    COA (206 KB)

    User Guide for Antibodies (1077 KB)

    AKT1+AKT2+AKT3 Antibody (YA635) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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