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AKT1+AKT2+AKT3 Antibody (YA635)

(Synonyms: AKT1,AKT2,AKT3)
5 Cited Publications
Customer Review

Based on 5 publication(s) in Google Scholar

AKT1+AKT2+AKT3 Antibody (YA635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AKT1+AKT2+AKT3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat, Hamster

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
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Publications Citing Use of MedChemExpress (MCE) AKT1+AKT2+AKT3 Antibody (YA635)

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5 Publications Citing Use of MCE AKT1+AKT2+AKT3 Antibody (YA635) (2)
WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

AKT1+AKT2+AKT3 Antibody (YA635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AKT1+AKT2+AKT3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Hamster
  • Observed Molecular Weight
    Observed band size: 56 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 56 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human AKT1/2/3 aa:281-480.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3694201

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and C6(lane 4(20μg) using AKT1/AKT2/AKT3 (HY-P80535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    P31749: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis (PubMed:11882383, PubMed:15526160, PubMed:15861136, PubMed:21432781, PubMed:21620960, PubMed:31204173). This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates (PubMed:11882383, PubMed:15526160, PubMed:21432781, PubMed:21620960, PubMed:29343641, PubMed:31204173).
    P31751: Serine/threonine kinase closely related to AKT1 and AKT3. All 3 enzymes, AKT1, AKT2 and AKT3, are collectively known as AKT kinase. AKT regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis, through the phosphorylation of a range of downstream substrates.
    Q9Y243: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates.

  • Subcellular Localization

    P31749: Cytoplasm; Nucleus; Cell membrane; Mitochondrion intermembrane space
    P31751: Cytoplasm; Nucleus; Cell membrane; Peripheral membrane protein; Early endosome
    Q9Y243: Nucleus; Cytoplasm; Membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity: P31749: Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH) , ductal carcinoma in situ (DCIS) , invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages
    P31751: Widely expressed. Expressed in myoblasts (PubMed:17565718)
    Q9Y243: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney

  • Subunit

    P31749: Interacts with BTBD10 (By similarity). Interacts with KCTD20 (By similarity). Interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10.
    P31751: Interacts with BTBD10 (By similarity).
    Q9Y243: Interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.

  • SwissProt ID

    P31749 / P31751 / Q9Y243

  • Gene ID
    207 [NCBI] / 208 [NCBI] / 10000 [NCBI]
  • Synonyms

    AKT1,AKT2,AKT3

  • Research Field

    Signal Transduction

AKT1+AKT2+AKT3 Antibody (YA635) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and C6(lane 4(20μg) using AKT1/AKT2/AKT3 (HY-P80535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    AKT1+AKT2+AKT3 Antibody (YA635) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jan:150:157741. [Abstract]

    Customer Validation
     A549 and LLC cells were treated with increasing doses of TXC for 24 h, followed by western blotting for ERBB2, PI3K, phospho-PI3K, AKT, phospho-AKT, mTOR, phospho-Mtor.

  • 1/16

    AKT1+AKT2+AKT3 Antibody (YA635) purchased from MedChemExpress. Usage Cited in: Viruses. 2025 Dec 17;17(12):1637. [Abstract]

    Customer Validation
     Quercetin suppresses the PI3K/Akt/mTOR signaling pathway. MARC-145 cells were infected with PRRSV (MOI of 0.8) and treated with quercetin for 48 h.

  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
    1/16
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and C6(lane 4(20μg) using AKT1/AKT2/AKT3 (HY-P80535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • Experimental Validation Results for AKT1+AKT2+AKT3 Antibody (YA635)
  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and C6(lane 4(20μg) using AKT1/AKT2/AKT3 (HY-P80535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling AKT1+AKT2+AKT3 with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AKT1+AKT2+AKT3 Antibody (HY-P80535) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image WB
  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image IHC-P
  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image IHC-P
  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image ICC
  • AKT1+AKT2+AKT3 Antibody (YA635) Product Image ICC