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FITC-conjugated Goat Anti-Rabbit IgG H&L

3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

FITC-conjugated Goat Anti-Rabbit IgG H&L is an FITC-conjugated,Goat-derived anti-Rabbit antibody. FITC-conjugated Goat Anti-Rabbit IgG H&L can binds to the IgG heavy and light chain of Rabbit antibodies.

For research use only. We do not sell to patients.
  • Host:

    Goat

  • Isotype:

    IgG

  • Application:

    ICC/IF, IF-Tissue, FC

  • Reactivity :

    Rabbit

  • Formulation:

    Supplied in PBS, pH7.4, containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    FITC
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • FITC-conjugated Goat Anti-Rabbit IgG H&L Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) FITC-conjugated Goat Anti-Rabbit IgG H&L

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3 Publications Citing Use of MCE FITC-conjugated Goat Anti-Rabbit IgG H&L (3)
IF
  • IF
  • IF
  • IF

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
Dilution Ratio 1:100-1000 1:100-1000 1:1000-4000

Product Details

Description

FITC-conjugated Goat Anti-Rabbit IgG H&L is an FITC-conjugated,Goat-derived anti-Rabbit antibody. FITC-conjugated Goat Anti-Rabbit IgG H&L can binds to the IgG heavy and light chain of Rabbit antibodies.

  • Host Goat
  • Clonality Polyclonal
  • Species Reactivity
    Rabbit
Immunogen

Synthetic peptide corresponding to Rabbit IgG.

Purification

Affinity Purified

Conjugation

FITC

Modification

Unmodified

Target Specificity

IgG heavy and light chain

Antibody Form

Whole IgG Antibodies

Minimal Cross Reactivity

Human, Mouse

Isotype

IgG

RRID

AB_3102768

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH7.4, containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Protect from light. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
    Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.

Background

  • Function

    Secondary antibodies are antibodies that bind to primary antibodies in immunoassays. They do not directly recognize the target antigen but rather the Fc region of the primary antibody or the entire immunoglobulin (Ig). Secondary antibodies are typically conjugated to fluorescent groups (such as Alexa Fluor), enzymes (such as HRP and alkaline phosphatase), or proteins (such as biotin).

  • Research Field

    Immunology

FITC-conjugated Goat Anti-Rabbit IgG H&L Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.

  • 1/16

    FITC-conjugated Goat Anti-Rabbit IgG H&L purchased from MedChemExpress. Usage Cited in: SSRN. 2026 Mar 7.

    Customer Validation
     Increased intestinal inflammation and weakened barrier function in adult zebrafish exposed to environmental concentrations of arecoline and arecaidine. Representative confocal immunofluorescence images.

  • 1/16

    FITC-conjugated Goat Anti-Rabbit IgG H&L purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    Customer Validation
     Cells with or without E2F4-GFP or E2F4-Myc plasmid transfection were fixed with 4% formaldehyde, blocked with 5% BSA, and subjected to IF staining. For cells not transfected or transfected with E2F4-Myc, anti-E2F4 (10923-1-AP, Proteintech, 1:20 dilution), anti-IgG (98136-1-RR, Proteintech, 1:50 dilution), and anti-Myc (16286-1-AP, Proteintech, 1:100 dilution) antibodies were used for staining. The primary antibody-incubated samples were incubated with goat anti-rabbit IgG H&L (FITC) (MCE, HY-P80951, 1:100 dilution).

  • 1/16

    FITC-conjugated Goat Anti-Rabbit IgG H&L purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2458184. [Abstract]

    Customer Validation
     Colon tissues fixed in 4% paraformaldehyde were embedded in paraffin and sectioned (4 μm). The sections were deparaffinized to water for antigen recovery. Sections were incubated at room temperature in the dark with 3% hydrogen peroxide solution for 25 minutes to block endogenous peroxidase activity. After blocking with 3% BSA, sections were incubated overnight at 4°C with rabbit anti-mouse IgA antibody (bs-0774 R, Bioss), rabbit polyclonal anti-C3 (21337–1-AP, Proteintech), and goat anti-mouse IgG H&L (FITC) (HY-P80950, MedChemExpress), then incubated with Goat Anti-Rabbit IgG H&L (FITC) (HY-P80951, MedChemExpress) at room temperature for 50 minutes.

  • FITC-conjugated Goat Anti-Rabbit IgG H&L Product Image
    1/16
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
  • FITC-conjugated Goat Anti-Rabbit IgG H&L Product Image
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L
    1/1
    MedchemExpress Validation
     Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.
  • Experimental Validation Results for FITC-conjugated Goat Anti-Rabbit IgG H&L

  • FITC-conjugated Goat Anti-Rabbit IgG H&L purchased from MedChemExpress. Usage Cited in: SSRN. 2026 Mar 7.

    Increased intestinal inflammation and weakened barrier function in adult zebrafish exposed to environmental concentrations of arecoline and arecaidine. Representative confocal immunofluorescence images.

  • FITC-conjugated Goat Anti-Rabbit IgG H&L purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    Cells with or without E2F4-GFP or E2F4-Myc plasmid transfection were fixed with 4% formaldehyde, blocked with 5% BSA, and subjected to IF staining. For cells not transfected or transfected with E2F4-Myc, anti-E2F4 (10923-1-AP, Proteintech, 1:20 dilution), anti-IgG (98136-1-RR, Proteintech, 1:50 dilution), and anti-Myc (16286-1-AP, Proteintech, 1:100 dilution) antibodies were used for staining. The primary antibody-incubated samples were incubated with goat anti-rabbit IgG H&L (FITC) (MCE, HY-P80951, 1:100 dilution).

  • FITC-conjugated Goat Anti-Rabbit IgG H&L purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2458184. [Abstract]

    Colon tissues fixed in 4% paraformaldehyde were embedded in paraffin and sectioned (4 μm). The sections were deparaffinized to water for antigen recovery. Sections were incubated at room temperature in the dark with 3% hydrogen peroxide solution for 25 minutes to block endogenous peroxidase activity. After blocking with 3% BSA, sections were incubated overnight at 4°C with rabbit anti-mouse IgA antibody (bs-0774 R, Bioss), rabbit polyclonal anti-C3 (21337–1-AP, Proteintech), and goat anti-mouse IgG H&L (FITC) (HY-P80950, MedChemExpress), then incubated with Goat Anti-Rabbit IgG H&L (FITC) (HY-P80951, MedChemExpress) at room temperature for 50 minutes.

  • FITC-conjugated Goat Anti-Rabbit IgG H&L Product Image
    MedchemExpress Validation

    Please select the appropriate fluorescent dye based on your fluorescence imaging system. According to the species of the experimental sample you are studying and the host species of the primary antibody, choose the appropriate species of the secondary antibody.

  • FITC-conjugated Goat Anti-Rabbit IgG H&L Product Image Others