phospho-PI3 Kinase p110 beta (Ser1070) Antibody

Cat. No.: HY-P81211: Data Sheet COA
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phospho-PI3 Kinase p110 beta (Ser1070) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to phospho-PI3 Kinase p110 beta (Ser1070).

For research use only. We do not sell to patients.

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phospho-PI3 Kinase p110 beta (Ser1070) Antibody Featured Recommendations:

6 Publications Citing Use of MCE phospho-PI3 Kinase p110 beta (Ser1070) Antibody

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Drug Des Devel Ther. 2025 Jan 17:19:349-368. [Abstract]; DCHD inhibited PANoptosis by regulating the PI3K/AKT/NF-κB signaling pathway.Analysis and quantification of TLR4, Myd88, p-IκBα, p-PI3K, p-AKT, and Bcl2 expression using Western blotting in RAW 264.7 Macrophages.

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2025 Sep 5:505:117545. [Abstract]; The proteins of the PI3K/Akt pathway were detected through western blot in MDA-MB-231 cells.

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2025 Sep 5:505:117545. [Abstract]; The proteins of the PI3K/Akt pathway were detected through western blot in BT-549 cells.

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2025 Sep 5:505:117545. [Abstract]; The proteins of the PI3K/Akt pathway and CYPJ were detected through Western blot in tumor tissues.

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]; Western blotting of the PI3K/AKT/mTOR pathway-related proteins in caski, siHa, C-33A cells.

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]; PI3K inhibitor LY294002 was added in APLN overexpressed C-33A cells. Western blotting of the PI3K/AKT/mTOR pathway-related proteins.

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]; APLN receptor inhibitor ML221 was added in APLN overexpressed C-33A cells. Western blotting of the PI3K/AKT/mTOR pathway-related proteins.

: phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MCE. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]; Western blotting of APLN and the PI3K/AKT/mTOR pathwayrelated proteins in APLN knockdown in CaSki cells.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

phospho-PI3 Kinase p110 beta (Ser1070) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to phospho-PI3 Kinase p110 beta (Ser1070).

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 110-123 kDa;

Observed band size: 110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat Predicted Reactivity: Chicken, Dog, Pig, Cow, Horse, GuineaPig

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P42338

Gene ID

5291 [NCBI]

Immunogen

KLH conjugated synthesised phosphopeptide derived from human PI3 Kinase p110 beta around the phosphorylation site of Ser1070: YR(p-S)

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL IHC-P mIHC

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

Function：Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Also has a protein kinase activity showing autophosphorylation (PubMed:12502714)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:general expression

Subunit：Heterodimer of a catalytic subunit PIK3CB and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interaction with PIK3R2 is required for nuclear localization and nuclear export.

RRID

AB_3103102

Database

Entrez Gene: 5291 Human ; 74769 Mouse ;

SwissProt: P42338 Human ; Q3U4Q1 Mouse ; Q8BTI9 Mouse ;

OMIM: 602925 Human

Synonyms

PI3-kinase p110 subunit beta; p-PI3Kβ(Ser1070); PI 3-kinase C2β; p110 BETA; p110Beta; Phosphatidylinositol 3 kinase catalytic beta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic beta polypeptide; PI3 kinase p110 subunit beta; PI3-kinase subunit beta; PI3K; PI3K beta; PI3K-beta; PI3Kbeta; PI3KCB; PIK3C1; Pik3cb; PK3CB_HUMAN; PtdIns 3 kinase p110; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; 5-bisphosphate 3-kinase catalytic subunit beta isoform.

Documentation

Select Batch:

Data Sheet (236 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

phospho-PI3 Kinase p110 beta (Ser1070) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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phospho-PI3 Kinase p110 beta (Ser1070) Antibody

phospho-PI3 Kinase p110 beta (Ser1070) Antibody Featured Recommendations:

6 Publications Citing Use of MCE phospho-PI3 Kinase p110 beta (Ser1070) Antibody

Product Detail

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Background

Documentation

phospho-PI3 Kinase p110 beta (Ser1070) Antibody Related Classifications

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