Copy Link Print Information

phospho-PI3 Kinase p110 beta (Ser1070) Antibody

(Synonyms: PI3-kinase p110 subunit beta; p-PI3Kβ(Ser1070); PI 3-kinase C2β; p110 BETA; p110Beta; Phosphatidylinositol 3 kinase catalytic beta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic beta polypeptide; PI3 kinase p110 subunit beta; PI3-kinase subunit beta; PI3K; PI3K beta; PI3K-beta; PI3Kbeta; PI3KCB; PIK3C1; Pik3cb; PK3CB_HUMAN; PtdIns 3 kinase p110; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; 5-bisphosphate 3-kinase catalytic subunit beta isoform.)
7 Cited Publications
Customer Review

Based on 7 publication(s) in Google Scholar

phospho-PI3 Kinase p110 beta (Ser1070) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to phospho-PI3 Kinase p110 beta (Ser1070).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC mIHC
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) phospho-PI3 Kinase p110 beta (Ser1070) Antibody

More
7 Publications Citing Use of MCE phospho-PI3 Kinase p110 beta (Ser1070) Antibody (8)
WB
  • WB
  • WB
  • WB
  • WB
  • WB
  • WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:100-500

Product Details

Description

phospho-PI3 Kinase p110 beta (Ser1070) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to phospho-PI3 Kinase p110 beta (Ser1070).

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Chicken,Dog,Pig,Cow,Horse,GuineaPig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110-123 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthesised phosphopeptide derived from human PI3 Kinase p110 beta around the phosphorylation site of Ser1070: YR(p-S)

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3103102

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Western blot analysis of extracts from K562 (lane2, 20μg), MCF7 (lane3, 20μg), Jurkat (lane4, 20μg) and Hela (lane5, 20μg) using phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    PI3 Kinase p110 beta is a Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Also has a protein kinase activity showing autophosphorylation[1][2].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:general expression

  • Subunit

    Heterodimer of a catalytic subunit PIK3CB and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interaction with PIK3R2 is required for nuclear localization and nuclear export.

  • SwissProt ID

    P42338

  • Gene ID
    5291 [NCBI]
  • Synonyms

    PI3-kinase p110 subunit beta; p-PI3Kβ(Ser1070); PI 3-kinase C2β; p110 BETA; p110Beta; Phosphatidylinositol 3 kinase catalytic beta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic beta polypeptide; PI3 kinase p110 subunit beta; PI3-kinase subunit beta; PI3K; PI3K beta; PI3K-beta; PI3Kbeta; PI3KCB; PIK3C1; Pik3cb; PK3CB_HUMAN; PtdIns 3 kinase p110; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; 5-bisphosphate 3-kinase catalytic subunit beta isoform.

phospho-PI3 Kinase p110 beta (Ser1070) Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), MCF7 (lane3, 20μg), Jurkat (lane4, 20μg) and Hela (lane5, 20μg) using phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Jan 17:19:349-368. [Abstract]

    Customer Validation
     DCHD inhibited PANoptosis by regulating the PI3K/AKT/NF-κB signaling pathway.Analysis and quantification of TLR4, Myd88, p-IκBα, p-PI3K, p-AKT, and Bcl2 expression using Western blotting in RAW 264.7 Macrophages.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2025 Dec:505:117545. [Abstract]

    Customer Validation
     The proteins of the PI3K/Akt pathway were detected through western blot in MDA-MB-231 cells.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2025 Dec:505:117545. [Abstract]

    Customer Validation
     The proteins of the PI3K/Akt pathway were detected through western blot in BT-549 cells.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2025 Dec:505:117545. [Abstract]

    Customer Validation
     The proteins of the PI3K/Akt pathway and CYPJ were detected through Western blot in tumor tissues.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]

    Customer Validation
     Western blotting of the PI3K/AKT/mTOR pathway-related proteins in caski, siHa, C-33A cells.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]

    Customer Validation
     PI3K inhibitor LY294002 was added in APLN overexpressed C-33A cells. Western blotting of the PI3K/AKT/mTOR pathway-related proteins.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]

    Customer Validation
     APLN receptor inhibitor ML221 was added in APLN overexpressed C-33A cells. Western blotting of the PI3K/AKT/mTOR pathway-related proteins.

  • 1/16

    phospho-PI3 Kinase p110 beta (Ser1070) Antibody purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2024 May:755:109983. [Abstract]

    Customer Validation
     Western blotting of APLN and the PI3K/AKT/mTOR pathwayrelated proteins in APLN knockdown in CaSki cells.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    1/16
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), MCF7 (lane3, 20μg), Jurkat (lane4, 20μg) and Hela (lane5, 20μg) using phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedChemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • Experimental Validation Results for phospho-PI3 Kinase p110 beta (Ser1070) Antibody
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane2, 20μg), MCF7 (lane3, 20μg), Jurkat (lane4, 20μg) and Hela (lane5, 20μg) using phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling phospho-PI3 Kinase p110 beta (Ser1070) with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with phospho-PI3 Kinase p110 beta (Ser1070) Antibody (HY-P81211) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedChemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image WB
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image IHC-P
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image IHC-P
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image IHC-P
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image ICC
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image ICC
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image mIHC
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image mIHC
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image mIHC
  • phospho-PI3 Kinase p110 beta (Ser1070) Antibody Product Image mIHC