Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody

Cat. No.: HY-P80846: Data Sheet COA
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Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199).

For research use only. We do not sell to patients.

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10 μL	In-stock	Estimated Time of Arrival: December 31
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Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody Featured Recommendations:

8 Publications Citing Use of MCE Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody

: Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody purchased from MCE. Usage Cited in: Int J Biol Macromol. 2026 Jan;335(Pt 1):149046. [Abstract]; Results of Western Blot analysis. They respectively display the relative expression levels of p-PI3K, p-AKT, and p-NFκB p65 proteins in CAOV3 and OVCAR3 cells (Cell lines were obtained as described in the Methods) when ITGB8 is knocked down compared with the negative control.

: Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody purchased from MCE. Usage Cited in: Phytomedicine. 2025 Dec 26:150:157741. [Abstract]; A549 and LLC cells were treated with increasing doses of TXC for 24 h, followed by western blotting for ERBB2, PI3K, phospho-PI3K, AKT, phospho-AKT, mTOR, phospho-Mtor.

: Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody purchased from MCE. Usage Cited in: FASEB J. 2025 Jun 15;39(11):e70695. [Abstract]; PI3K and AKT phosphorylation, and NRF2 expression in CD44KO erythroid cells.

: Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody purchased from MCE. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]; The effects of OGT on cell proliferation, metastasis, and apoptosis partially depend upon the PI3K/AKT/mTOR signalling pathway in OSCC cells. Levels of PI3K, AKT, mTOR, p-PI3K, p-AKT, p-mTOR, OGT, and O-GlcNAcylation proteins were assessed in OSCC cells exposed to a medium with different glucose concentrations and OGT knockdown.

: Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody purchased from MCE. Usage Cited in: Viruses. 2025 Dec 17;17(12):1637. [Abstract]; Quercetin suppresses the PI3K/Akt/mTOR signaling pathway. MARC-145 cells were infected with PRRSV (MOI of 0.8) and treated with quercetin for 48 h.

: Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody purchased from MCE. Usage Cited in: Endocrine. 2024 Jun;84(3):1051-1063. [Abstract]; WB detectd the expression of PI3K/Akt pathway and downstream target molecules in K1 and CAL-62 transfected with sh-ID2, Ctrl, or oe-ID2. After MK-2206 (AKT inhibitor) treatment.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199).

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 84 kDa; Observed band size: 55,85 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P27986

Gene ID

5295 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr467/199 of Human PI3-kinase p85-alpha/gamma.AA range:436-485.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

1.Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
2.Supplied in PBS containing 150 mM NaCl, 50% glycerol and 0.02% sodium azid, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from HEK293(lane 2(40μg) and HEK293(lane 3(80μg) using Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) (HY-P80790) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-PI3 Kinase p85 with Phospho-PI3 Kinase p85 antibody (HY-P80846) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PI3 Kinase p85 antibody (HY-P80970) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-PI3 Kinase p85 with Phospho-PI3 Kinase p85 antibody (HY-P80846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PI3 Kinase p85 antibody (HY-P80846) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923)

Expression：
Tissue_specificity:Isomer 2 is expressed in skeletal muscle and brain tissue, and at lower levels in the kidneys and cardiac muscle. Both isomer 2 and isomer 4 are present in skeletal muscle (protein level) .

Isoforms & Post-Translational Modification：P27986 has 5 isomers: P27986-1: 83598 Da (predicted); P27986-2: 53486 Da (predicted); P27986-3: 49965 Da (predicted); P27986-4: 84474 Da (predicted); P27986-5: 42838 Da (predicted).
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation;In adipose tissue, polyubiquitinated by the BCR(KBTBD2) E3 ubiquitin ligase complex; recognized by KBTBD2 through the SH2 domains, undergoes 'Lys-48'-linked polyubiquitination leading to its degradation;Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR

Subunit：Heterodimer of a regulatory subunit PIK3R1 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts (via SH2 domains) with CCDC88A/GIV (tyrosine-phosphorylated form); the interaction enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration (PubMed:21954290). Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with PIK3R2; the interaction is dissociated in an insulin-dependent manner (By similarity). Interacts with XBP1 isoform 2; the interaction is direct and induces translocation of XBP1 isoform 2 into the nucleus in a ER stress- and/or insulin-dependent but PI3K-independent manner (PubMed:20348923). Interacts with FER. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Interacts with PTK2/FAK1 (By similarity). Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with LYN (via SH3 domain); this enhances enzyme activity (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1, IRS2 and phosphorylated IRS4, as well as with NISCH and HCST (PubMed:8628286, PubMed:19109239). Interacts with FASLG, KIT and BCR. Interacts with AXL, FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with FGR and HCK. Interacts with PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with ERBB4 (phosphorylated). Interacts with NTRK1 (phosphorylated upon ligand-binding). Interacts with FAM83B; activates the PI3K/AKT signaling cascade (PubMed:23676467). Interacts with APPL1 and APPL2 (By similarity). Interacts with SRC (PubMed:28903391). Interacts with ALOX5; this interaction bridges ALOX5 with CD40 after CD40 ligation in B cells and leads to the production of reactive oxygen species (ROS) (PubMed:21200133). Interacts with TYK2 (PubMed:10995743). Interacts with nephrin NPHN1; the interaction is reduced by high glucose levels (PubMed:28955049). Interacts with CASP8 (phosphorylated on Tyr-380) (PubMed:27109099). Interacts with CD28 (PubMed:7568038). Interacts with ICOS (PubMed:30523347)

RRID

AB_3102229

Database

Entrez Gene: 5295 Human ; 18708 Mouse ; 25513 Rat

SwissProt: P27986 Human ; P26450 Mouse ; Q63787 Rat

OMIM: 615214 Human

Research Field

Signal Transduction

Synonyms

PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase s

Documentation

Select Batch:

Data Sheet (235 KB) SDS (313 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody

Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody Featured Recommendations:

8 Publications Citing Use of MCE Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody

Product Detail

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Background

Documentation

Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody Related Classifications

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