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Phospho-mTOR (Ser2448) Antibody (YA171)

(Synonyms: MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Phospho-mTOR (Ser2448) Antibody (YA171) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-mTOR (Ser2448).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL IHC-P mIHC
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
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Top Publications Citing

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Publications Citing Use of MedChemExpress (MCE) Phospho-mTOR (Ser2448) Antibody (YA171)

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2 Publications Citing Use of MCE Phospho-mTOR (Ser2448) Antibody (YA171) (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Phospho-mTOR (Ser2448) Antibody (YA171) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-mTOR (Ser2448).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 289 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 289 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser2448 of Human mTOR.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102221

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    mTOR (mammalian target of Rapamycin) is a protein that in humans is encoded by the mTOR gene. mTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. mTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. mTOR integrates the input from upstream pathways, including growth factors and amino acids. mTOR also senses cellular nutrient, oxygen, and energy levels. The mTOR pathway is dysregulated in human diseases, such as diabetes, obesity, depression, and certain cancers. Rapamycin inhibits mTOR by associating with its intracellular receptor FKBP12. The FKBP12-rapamycin complex binds directly to the FKBP12-Rapamycin Binding (FRB) domain of mTOR, inhibiting its activity.

  • Subcellular Localization

    Lysosome membrane; Peripheral membrane protein; Cytoplasmic side; Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side; Cell membrane; Peripheral membrane protein; Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm; Nucleus; Nucleus, PML body; Microsome membrane; Cytoplasmic vesicle, phagosome

  • Expression


    Tissue_specificity:It is expressed in multiple tissues, with the highest concentration in the testes.

  • Subunit

    Part of the mechanistic target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8 and RPTOR

  • SwissProt ID

    P42345

  • Gene ID
    2475 [NCBI]
  • Synonyms

    MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar

  • Research Field

    Cell Biology

[1]. Laplante M, et al. mTOR signaling in growth control and disease. Cell. 2012 Apr 13;149(2):274-93. [Content Brief]

[2]. Panwar V, et al. Multifaceted role of mTOR (mammalian target of rapamycin) signaling pathway in human health and disease. Signal Transduct Target Ther. 2023 Oct 2;8(1):375. [Content Brief]

[3]. Yong J, et al. Regulation of transcriptome plasticity by mTOR signaling pathway. Exp Mol Med. 2025 Aug;57(8):1623-1630. [Content Brief]

[4]. Ballesteros-Álvarez J, et al. mTORC2: The other mTOR in autophagy regulation. Aging Cell. 2021 Aug;20(8):e13431. [Content Brief]

[5]. Patel CH, et al. More TOR: The expanding role of mTOR in regulating immune responses. Immunity. 2025 Jul 8;58(7):1629-1645. [Content Brief]

[6]. Dai DF, et al. The mTOR signaling pathway in cardiac aging. J Cardiovasc Aging. 2023;3(3):24. [Content Brief]

[7]. Wang F, et al. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. Nat Commun. 2018 Nov 19;9(1):4874. [Content Brief]

[8]. Schaub T, et al. mTORC1 and mTORC2 Differentially Regulate Cell Fate Programs to Coordinate Osteoblastic Differentiation in Mesenchymal Stromal Cells. Sci Rep. 2019 Dec 27;9(1):20071. [Content Brief]

[9]. Mao B, et al. Overview of Research into mTOR Inhibitors. Molecules. 2022 Aug 19;27(16):5295. [Content Brief]

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Phospho-mTOR (Ser2448) Antibody (YA171) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    Phospho-mTOR (Ser2448) Antibody (YA171) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jan:150:157741. [Abstract]

    Customer Validation
     A549 and LLC cells were treated with increasing doses of TXC for 24 h, followed by western blotting for ERBB2, PI3K, phospho-PI3K, AKT, phospho-AKT, mTOR, phospho-Mtor.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    1/16
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)
  • Experimental Validation Results for Phospho-mTOR (Ser2448) Antibody (YA171)

  • Phospho-mTOR (Ser2448) Antibody (YA171) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jan:150:157741. [Abstract]

    A549 and LLC cells were treated with increasing doses of TXC for 24 h, followed by western blotting for ERBB2, PI3K, phospho-PI3K, AKT, phospho-AKT, mTOR, phospho-Mtor.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image IHC-P
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image IHC-P
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image IHC-P
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image IHC-P
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image IHC-P
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image IHC-P
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image mIHC
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image mIHC
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image mIHC
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image mIHC
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image mIHC
  • Phospho-mTOR (Ser2448) Antibody (YA171) Product Image mIHC