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mTOR Antibody (YA281)

(Synonyms: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Tyrosine-protein kinase mTOR, mTOR)
3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

mTOR Antibody (YA281) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to mTOR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • mTOR Antibody (YA281) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

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Publications Citing Use of MedChemExpress (MCE) mTOR Antibody (YA281)

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3 Publications Citing Use of MCE mTOR Antibody (YA281) (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000 1:200-1:1000 1:50-1:200 1:500 Use at an assay dependent concentration.

Product Details

Description

mTOR Antibody (YA281) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to mTOR.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 289 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 289 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human mTOR.AA range:2400-2440.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102364

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for mTOR Antibody (YA281)
    Western blot analysis of extracts from Hela (lane 2(20μg), HepG2 (lane 3(20μg), HEK-293 (lane 4(20μg) using mTOR Antibody (HY-P80231) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80231, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for mTOR Antibody (YA281)
    Western blot analysis of extracts from Hela(lane 2(20ug) , MCF-7(lane 3(20ug) and HepG2(lane 4(20ug) using mTORAntibody (HY-P80231) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for mTOR Antibody (YA281)
    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for mTOR Antibody (YA281)
    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    mTOR (mammalian target of Rapamycin) is a protein that in humans is encoded by the mTOR gene. mTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. mTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. mTOR integrates the input from upstream pathways, including growth factors and amino acids. mTOR also senses cellular nutrient, oxygen, and energy levels. The mTOR pathway is dysregulated in human diseases, such as diabetes, obesity, depression, and certain cancers. Rapamycin inhibits mTOR by associating with its intracellular receptor FKBP12. The FKBP12-rapamycin complex binds directly to the FKBP12-Rapamycin Binding (FRB) domain of mTOR, inhibiting its activity.

  • Subcellular Localization

    Lysosome membrane; Peripheral membrane protein; Cytoplasmic side; Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side; Cell membrane; Peripheral membrane protein; Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm; Nucleus; Nucleus, PML body; Microsome membrane; Cytoplasmic vesicle, phagosome

  • Expression


    Tissue_specificity:It is expressed in multiple tissues, with the highest concentration in the testes.

  • Subunit

    Part of the mechanistic target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8 and RPTOR

  • SwissProt ID

    P42345

  • Gene ID
    2475 [NCBI]
  • Synonyms

    FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Tyrosine-protein kinase mTOR, mTOR

  • Research Field

    Cell Biology

[1]. Laplante M, et al. mTOR signaling in growth control and disease. Cell. 2012 Apr 13;149(2):274-93. [Content Brief]

[2]. Panwar V, et al. Multifaceted role of mTOR (mammalian target of rapamycin) signaling pathway in human health and disease. Signal Transduct Target Ther. 2023 Oct 2;8(1):375. [Content Brief]

[3]. Yong J, et al. Regulation of transcriptome plasticity by mTOR signaling pathway. Exp Mol Med. 2025 Aug;57(8):1623-1630. [Content Brief]

[4]. Ballesteros-Álvarez J, et al. mTORC2: The other mTOR in autophagy regulation. Aging Cell. 2021 Aug;20(8):e13431. [Content Brief]

[5]. Patel CH, et al. More TOR: The expanding role of mTOR in regulating immune responses. Immunity. 2025 Jul 8;58(7):1629-1645. [Content Brief]

[6]. Dai DF, et al. The mTOR signaling pathway in cardiac aging. J Cardiovasc Aging. 2023;3(3):24. [Content Brief]

[7]. Wang F, et al. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. Nat Commun. 2018 Nov 19;9(1):4874. [Content Brief]

[8]. Schaub T, et al. mTORC1 and mTORC2 Differentially Regulate Cell Fate Programs to Coordinate Osteoblastic Differentiation in Mesenchymal Stromal Cells. Sci Rep. 2019 Dec 27;9(1):20071. [Content Brief]

[9]. Mao B, et al. Overview of Research into mTOR Inhibitors. Molecules. 2022 Aug 19;27(16):5295. [Content Brief]

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mTOR Antibody (YA281) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HepG2 (lane 3(20μg), HEK-293 (lane 4(20μg) using mTOR Antibody (HY-P80231) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80231, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , MCF-7(lane 3(20ug) and HepG2(lane 4(20ug) using mTORAntibody (HY-P80231) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    mTOR Antibody (YA281) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jan:150:157741. [Abstract]

    Customer Validation
     A549 and LLC cells were treated with increasing doses of TXC for 24 h, followed by western blotting for ERBB2, PI3K, phospho-PI3K, AKT, phospho-AKT, mTOR, phospho-Mtor.

  • mTOR Antibody (YA281) Product Image
    1/16
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • mTOR Antibody (YA281) Product Image
  • Experimental Validation Results for mTOR Antibody (YA281)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HepG2 (lane 3(20μg), HEK-293 (lane 4(20μg) using mTOR Antibody (HY-P80231) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80231, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for mTOR Antibody (YA281)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , MCF-7(lane 3(20ug) and HepG2(lane 4(20ug) using mTORAntibody (HY-P80231) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for mTOR Antibody (YA281)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for mTOR Antibody (YA281)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • Experimental Validation Results for mTOR Antibody (YA281)
  • mTOR Antibody (YA281) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), HepG2 (lane 3(20μg), HEK-293 (lane 4(20μg) using mTOR Antibody (HY-P80231) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80231, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • mTOR Antibody (YA281) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , MCF-7(lane 3(20ug) and HepG2(lane 4(20ug) using mTORAntibody (HY-P80231) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • mTOR Antibody (YA281) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • mTOR Antibody (YA281) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using mTOR Antibody (YA281). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80231, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • mTOR Antibody (YA281) Product Image WB
  • mTOR Antibody (YA281) Product Image WB
  • mTOR Antibody (YA281) Product Image IHC-P
  • mTOR Antibody (YA281) Product Image IHC-P