Oct-3/4 Antibody (YA4996)
(Synonyms: POU5F1; OCT3; OCT4; OTF3; POU domain; class 5, transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3)Oct-3/4 Antibody (YA4996) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Oct-3/4.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
WB; ICC/IF
-
Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-200 |
Product Details
Oct-3/4 Antibody (YA4996) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Oct-3/4.
-
Host Mouse
-
Clonality Monoclonal
-
Calculated Molecular Weight Predicted band size: 39 kDa;
KLH conjugated synthetic peptide derived from human OCT4. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Oct3/4 (also known as POU5F1 and Oct4) is regarded as one of the key regulators of pluripotency. Oct-4 is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. it is frequently used as a marker for undifferentiated cells. Oct-4 expression must be closely regulated; too much or too little will cause differentiation of the cells.
-
Subcellular Localization
Cytoplasm; Nucleus
-
Expression
Tissue_specificity:It is expressed in the developing brain. The highest expression levels are found in specific cell layers of the cerebral cortex, olfactory bulb, hippocampus, and cerebellum. Expression levels are lower in adult tissues.
Induction:Transcriptional activity is positively regulated by PKM (PubMed:18191611) . Upon cell differentiation, expression is repressed by NR6A1 (PubMed:26769970) -
Isoforms & Post-Translational Modification
Q01860 has 2 isomers: Q01860-1: 38571 Da (predicted); Q01860-2: 30085 Da (predicted).
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression;Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation;ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription -
Subunit
Interacts with PKM. Interacts with WWP2. Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (By similarity). Interacts with ZNF322 (By similarity). Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-355 (By similarity). Interacts (when phosphorylated on Ser-355) with FBXW8 (By similarity). Interacts with FBXW4 (By similarity). Interacts with SOX2 and SOX15; binds synergistically with either SOX2 or SOX15 to DNA (By similarity). Interacts with DDX56 (By similarity)
-
SwissProt ID
-
Synonyms
POU5F1; OCT3; OCT4; OTF3; POU domain; class 5, transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3
Documentation
Powered by Bioz
