2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. Protein Phosphatase 4C Antibody (YA687)

Protein Phosphatase 4C Antibody (YA687)

Cat. No.: HY-P80873
User Guide for Antibodies Technical Support

Protein Phosphatase 4C Antibody (YA687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Protein Phosphatase 4C.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Protein Phosphatase 4C Antibody (YA687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Protein Phosphatase 4C.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 35 kDa;
Observed band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human Protein Phosphatase 4C.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Sensitivity Endogenous Purity affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG2a  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin
Subcellular Localization：Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Subunit：Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4 (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2 (PubMed:12668731). Interacts with REL, NFKB1/p50 and RELA (PubMed:1336397). Interacts with SMN1 and GEMIN4 (PubMed:12668731). Interacts with IRS4 (phosphorylated) (PubMed:15331607). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2 (PubMed:16085932). Interacts with HDAC3 (PubMed:15805470)
Database

Entrez Gene: 5531 Human

SwissProt: P60510 Human

OMIM: 602035 Human

Research Field

Signal Transduction
Synonyms
PP4; PPX; PP4C; PPH3; PPP4
Documentation
User Guide for Antibodies (1077 KB)

Protein Phosphatase 4C Antibody (YA687) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Protein Phosphatase 4C Antibody (YA687)
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