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Protein Phosphatase 4C Antibody (YA687)

(Synonyms: PP4; PPX; PP4C; PPH3; PPP4)
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Based on 1 Customer Validation

Protein Phosphatase 4C Antibody (YA687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Protein Phosphatase 4C.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Protein Phosphatase 4C Antibody (YA687)
  • Protein Phosphatase 4C Antibody (YA687) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
Predicted band size Info
 
Observed band size Info
 
Dilution Ratio 35 kDa 35 kDa

Product Details

Description

Protein Phosphatase 4C Antibody (YA687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Protein Phosphatase 4C.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database

Entrez Gene: 5531 Human

SwissProt: P60510 Human

Immunogen

Synthetic peptide corresponding to Human Protein Phosphatase 4C.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Protein Phosphatase 4C Antibody (YA687)
    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2) using Protein Phosphatase 4C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Subunit

    Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4 (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2 (PubMed:12668731). Interacts with REL, NFKB1/p50 and RELA (PubMed:1336397). Interacts with SMN1 and GEMIN4 (PubMed:12668731). Interacts with IRS4 (phosphorylated) (PubMed:15331607). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2 (PubMed:16085932). Interacts with HDAC3 (PubMed:15805470)

  • SwissProt ID

    P60510

  • Gene ID
    5531 [NCBI]
  • Synonyms

    PP4; PPX; PP4C; PPH3; PPP4

  • Research Field

    Signal Transduction

Protein Phosphatase 4C Antibody (YA687) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2) using Protein Phosphatase 4C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Protein Phosphatase 4C Antibody (YA687) Product Image
    1/16
  • Experimental Validation Results for Protein Phosphatase 4C Antibody (YA687)
  • Protein Phosphatase 4C Antibody (YA687) Product Image
  • Experimental Validation Results for Protein Phosphatase 4C Antibody (YA687)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2) using Protein Phosphatase 4C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Protein Phosphatase 4C Antibody (YA687)
  • Protein Phosphatase 4C Antibody (YA687) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2) using Protein Phosphatase 4C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Protein Phosphatase 4C Antibody (YA687) Product Image WB