ANXA5 Antibody (YA7353)
(Synonyms: ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha)ANXA5 Antibody (YA7353) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ANXA5.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
ANXA5 Antibody (YA7353) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ANXA5.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 36 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 36 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Annexin A5 (AnxA5, Annexin V) is a Ca2+-dependent phosphatidylserine (PS) -binding protein that functions at the interface of membrane biology, apoptosis, coagulation, and inflammation[1][2]. Through its high-affinity interaction with exposed PS, AnxA5 regulates cellular responses associated with apoptotic cell clearance and membrane remodeling, while also modulating procoagulant phospholipid surfaces[1][3]. Mechanistically, extracellular AnxA5 forms organized assemblies on PS-expressing membranes and participates in biological processes including apoptosis, phagocytosis, blood coagulation, and microparticle formation[3][4]. In disease-relevant settings, AnxA5 suppresses endothelial inflammatory responses induced by activated platelets and microvesicles through PS-dependent mechanisms, supporting its role in vascular inflammation and sepsis-related pathology[5]. AnxA5 has also emerged as a widely used biomarker and molecular probe for the detection of apoptosis because externalization of PS is an early hallmark of apoptotic cells and can be specifically recognized by annexin-based assays[6][7]. Compared with other annexin family members, AnxA5 is distinguished by its extensive experimental application as a PS-targeting molecule for apoptosis detection, molecular imaging, and investigation of PS-mediated signaling pathways[2][6][8]. Structural studies further demonstrate that efficient PS binding and anticoagulant activity depend on intact AnxA5 membrane-interaction domains, highlighting the importance of PS recognition for its biological and research functions[9]. Recombinant human AnxA5 is therefore broadly employed as an experimental reagent for studying apoptosis, inflammation, coagulation, and membrane-associated pathophysiology[5][10].
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Isoforms & Post-Translational Modification
P08758: 320 amino acids, molecular weight 35937 Da.
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Subunit
Monomer
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SwissProt ID
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Synonyms
ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha
Documentation
References
[1]. Jing J. The Relevance, et al. Int J Mol Sci. 2024 Mar 1;25(5):2865.
[2]. Gerke V, et al. Annexins-a family of proteins with distinctive tastes for cell signaling and membrane dynamics. Nat Commun. 2024 Feb 21;15(1):1574. [Content Brief]
[3]. Rabezanahary H, et al. Live virus neutralizing antibodies against pre and post Omicron strains in food and retail workers in Québec, Canada. Heliyon. 2024 May 21;10(10):e31026. [Content Brief]
[4]. Reutelingsperger CP, et al. Annexin V, the regulator of phosphatidylserine-catalyzed inflammation and coagulation during apoptosis. Cell Mol Life Sci. 1997 Jun;53(6):527-32. [Content Brief]
[5]. Tschirhart BJ, et al. Annexin A5 Inhibits Endothelial Inflammation Induced by Lipopolysaccharide-Activated Platelets and Microvesicles via Phosphatidylserine Binding. Pharmaceuticals (Basel). 2023 Jun 3;16(6):837. [Content Brief]
[6]. van Engeland M, et al. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry. 1998 Jan 1;31(1):1-9. [Content Brief]
[7]. Liu T, et al. Detection of apoptosis based on the interaction between annexin V and phosphatidylserine. Anal Chem. 2009 Mar 15;81(6):2410-3. [Content Brief]
[8]. Laufer EM, et al. Annexin A5: an imaging biomarker of cardiovascular risk. Basic Res Cardiol. 2008 Mar;103(2):95-104. [Content Brief]
[9]. Wang J, et al. Domain IV of Annexin A5 Is Critical for Binding Calcium and Guarantees Its Maximum Binding to the Phosphatidylserine Membrane. Molecules. 2017 Dec 19;22(12):2256. [Content Brief]
[10]. Tschirhart BJ, et al. Pharmacokinetics of recombinant human annexin A5 (SY-005) in patients with severe COVID-19. Front Pharmacol. 2024 Jan 10;14:1299613. [Content Brief]
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