SYVN1 Antibody (YA6784)

Cat. No.: HY-P87091: Data Sheet COA
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SYVN1 Antibody (YA6784) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SYVN1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

SYVN1 Antibody (YA6784) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SYVN1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q86TM6

Gene ID

84447 [NCBI]

Immunogen

Synthetic peptide within Human SYVN1 aa 150-250.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、HepG2 (lane 3(20μg)) and A549 (lane 4(20μg)) using SYVN1 Antibody (HY-P87091) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:27827840, PubMed:26471130, PubMed:28827405). Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:26471130, PubMed:28842558). Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation (PubMed:17141218). Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis (PubMed:17170702). Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 (By similarity). During the early stage of B cell development, required for degradation of the pre-B cell receptor (pre-BCR) complex, hence supporting further differentiation into mature B cells (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane

Expression：
Tissue_Specificity: Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level)
Induction: By endoplasmic reticulum stress-inducing agents such as thapsigargin, tunicamycin or brefeldin A, but not by heat shock

Isoforms & Post-Translational Modification：Q86TM6 has three isomers: Q86TM6-1: 67685 Da (predicted); Q86TM6-2: 61732 Da (predicted); Q86TM6-3: 67614 Da (predicted).
Not N-glycosylated丨Auto-ubiquitinated

Subunit：Homodimer (PubMed:16289116)

Synonyms

1200010C09Rik antibody; DER3 antibody; E3 ubiquitin-protein ligase synoviolin antibody; HMG coA reductase degradation 1 homolog antibody; HRD1 antibody; KIAA1810 antibody; MGC40372 antibody; OTTHUMP00000230429 antibody; OTTHUMP00000230430 antibody; OTTHUMP00000230431 antibody; 1200010C09Rik antibody; DER3 antibody; E3 ubiquitin-protein ligase synoviolin antibody; HMG coA reductase degradation 1 homolog antibody; HRD1 antibody; KIAA1810 antibody; MGC40372 antibody; OTTHUMP00000230429 antibody; OTTHUMP00000230430 antibody; OTTHUMP00000230431 antibody; OTTHUMP00000230432 antibody; Synovial apoptosis inhibitor 1 antibody; Synovial apoptosis inhibitor 1, synoviolin antibody; Synoviolin 1 antibody; Synoviolin 1 isoform b antibody; SYNOVIOLIN antibody; SYVN1 antibody; SYVN1_HUMAN antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)