OPA1 Antibody (YA7541)

製品番号: HY-P87856: データシート User Guide for Antibodies
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OPA1 Antibody (YA7541) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to OPA1.

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容量		在庫状況
50 μL		お問い合わせ
100 μL		お問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

OPA1 Antibody (YA7541) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to OPA1.

Host

Rabbit

Clonality

Monoclonal,Recombinant

分子量

Predicted band size: 112 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O60313

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:4000
WB WB: Western Blot	1:10000-1:50000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

Sensitivity	Endogenous	純度	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG,Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Dynamin-related GTPase that is essential for normal mitochondrial morphology by mediating fusion of the mitochondrial inner membranes, regulating cristae morphology and maintaining respiratory chain function (PubMed:16778770, PubMed:17709429, PubMed:20185555, PubMed:24616225, PubMed:28628083, PubMed:28746876, PubMed:31922487, PubMed:32228866, PubMed:32567732, PubMed:33130824, PubMed:33237841, PubMed:37612504, PubMed:37612506). Exists in two forms: the transmembrane, long form (Dynamin-like GTPase OPA1, long form; L-OPA1), which is tethered to the inner mitochondrial membrane, and the short soluble form (Dynamin-like GTPase OPA1, short form; S-OPA1), which results from proteolytic cleavage and localizes in the intermembrane space (PubMed:31922487, PubMed:32228866, PubMed:33237841, PubMed:37612504, PubMed:37612506). Both forms (L-OPA1 and S-OPA1) cooperate to catalyze the fusion of the mitochondrial inner membrane (PubMed:31922487, PubMed:37612504, PubMed:37612506). The equilibrium between L-OPA1 and S-OPA1 is essential: excess levels of S-OPA1, produced by cleavage by OMA1 following loss of mitochondrial membrane potential, lead to an impaired equilibrium between L-OPA1 and S-OPA1, inhibiting mitochondrial fusion (PubMed:20038677, PubMed:31922487).

Subcellular Localization：Mitochondrion inner membrane,Mitochondrion intermembrane space

Expression：
Tissue_Specificity: Highly expressed in retina (PubMed:11017079, PubMed:11017080, PubMed:11810270). Also expressed in brain, testis, heart and skeletal muscle (PubMed:11810270). Low levels of all isoforms expressed in a variety of tissues (PubMed:11810270)|Expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Low levels of all isoforms expressed in a variety of tissues|Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes

Isoforms & Post-Translational Modification：O60313 has 6 isomers: O60313-1: 111631 Da (predicted); O60313-2: 115884 Da (predicted); O60313-9: 111822 Da (predicted); O60313-10: 117744 Da (predicted); O60313-11: 113490 Da (predicted); O60313-13: 107568 Da (predicted).
Cleaved by OMA1 or YME1L downstream of the transmembrane region in response to different signals to generate soluble forms (PubMed:16778770, PubMed:17709429, PubMed:20038677, PubMed:24616225, PubMed:27495975, PubMed:33237841)丨Cleavage at position S2 by YME1L is required to mediate oxidative phosphorylation (OXPHOS)-induced mitochondrial fusion (PubMed:17709429, PubMed:24616225, PubMed:27495975)丨Cleavage at position S2 by YME1L is required to mediate oxidative phosphorylation (OXPHOS)-induced mitochondrial fusion (PubMed:17709429, PubMed:24616225, PubMed:27495975)丨Cleavage at position S2 by YME1L is required to mediate oxidative phosphorylation (OXPHOS)-induced mitochondrial fusion (PubMed:17709429, PubMed:24616225, PubMed:27495975)丨Cleavage at position S3 by YME1L is required for membrane tubulation

Subunit：Oligomeric complex consisting of membrane-bound and soluble forms of OPA1 (PubMed:20185555, PubMed:37612504, PubMed:37612506)

ドキュメンテーション

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/molm-13-cell.html [Content Brief]