OPA1 Antibody (YA7541)
OPA1 Antibody (YA7541) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to OPA1.
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Host:
Rabbit
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Isotype:
IgG,Kappa
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:4000 | 1:10000-1:50000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
OPA1 Antibody (YA7541) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to OPA1.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 80 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 112 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG,Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
OPA1 is a Dynamin-related GTPase that is essential for normal mitochondrial morphology by mediating fusion of the mitochondrial inner membranes, regulating cristae morphology and maintaining respiratory chain function. Exists in two forms: the transmembrane, long form (Dynamin-like GTPase OPA1, long form; L-OPA1), which is tethered to the inner mitochondrial membrane, and the short soluble form (Dynamin-like GTPase OPA1, short form; S-OPA1), which results from proteolytic cleavage and localizes in the intermembrane space. Both forms (L-OPA1 and S-OPA1) cooperate to catalyze the fusion of the mitochondrial inner membrane. The equilibrium between L-OPA1 and S-OPA1 is essential: excess levels of S-OPA1, produced by cleavage by OMA1 following loss of mitochondrial membrane potential, lead to an impaired equilibrium between L-OPA1 and S-OPA1, inhibiting mitochondrial fusion[1][2][3][4][5][6][7][8][9][10][11][12][13][14].
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Subcellular Localization
Mitochondrion inner membrane,Mitochondrion intermembrane space
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Expression
Tissue_Specificity: Highly expressed in retina (PubMed:11017079, PubMed:11017080, PubMed:11810270). Also expressed in brain, testis, heart and skeletal muscle (PubMed:11810270). Low levels of all isoforms expressed in a variety of tissues (PubMed:11810270)|Expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Low levels of all isoforms expressed in a variety of tissues|Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes -
Isoforms & Post-Translational Modification
O60313 has 6 isomers: O60313-1: 111631 Da (predicted); O60313-2: 115884 Da (predicted); O60313-9: 111822 Da (predicted); O60313-10: 117744 Da (predicted); O60313-11: 113490 Da (predicted); O60313-13: 107568 Da (predicted).
Cleaved by OMA1 or YME1L downstream of the transmembrane region in response to different signals to generate soluble forms (PubMed:16778770, PubMed:17709429, PubMed:20038677, PubMed:24616225, PubMed:27495975, PubMed:33237841)丨Cleavage at position S2 by YME1L is required to mediate oxidative phosphorylation (OXPHOS)-induced mitochondrial fusion (PubMed:17709429, PubMed:24616225, PubMed:27495975)丨Cleavage at position S2 by YME1L is required to mediate oxidative phosphorylation (OXPHOS)-induced mitochondrial fusion (PubMed:17709429, PubMed:24616225, PubMed:27495975)丨Cleavage at position S2 by YME1L is required to mediate oxidative phosphorylation (OXPHOS)-induced mitochondrial fusion (PubMed:17709429, PubMed:24616225, PubMed:27495975)丨Cleavage at position S3 by YME1L is required for membrane tubulation -
Subunit
Oligomeric complex consisting of membrane-bound and soluble forms of OPA1 (PubMed:20185555, PubMed:37612504, PubMed:37612506)
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SwissProt ID
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