Prostaglandin D Synthase Antibody (YA1739)(PBS only)
(Synonyms: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS)Prostaglandin D Synthase Antibody (YA1739) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostaglandin D Synthase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:100 | 1:50-1:200 |
Product Details
Prostaglandin D Synthase Antibody (YA1739) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Prostaglandin D Synthase.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 26 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 21 kDa
A synthetic peptide of human Prostaglandin D Synthase
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HeLa(lane 2(20μg), HepG2(lane 3(20μg), THP-1(lane 4(20μg), RAW 264.7(lane 5(20μg) using Prostaglandin D Synthase Antibody (HY-P81994). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue using Prostaglandin D Synthase Antibody (YA1739). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81994, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human glioma tissue using Prostaglandin D Synthase Antibody (YA1739). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81994, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Prostaglandin D Synthase Antibody (YA1739). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81994, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunocytochemistry analysis of HepG2 cells labeling Prostaglandin D Synthase with Prostaglandin D Synthase Antibody (HY-P81994) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Prostaglandin D Synthase Antibody (HY-P81994) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of HepG2 cells labeling Prostaglandin D Synthase with Prostaglandin D Synthase Antibody (HY-P81994) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Prostaglandin D Synthase Antibody (HY-P81994) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of HepG2 cells labeling Prostaglandin D Synthase with Prostaglandin D Synthase Antibody (HY-P81994) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Prostaglandin D Synthase Antibody (HY-P81994) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of HepG2 cells labeling Prostaglandin D Synthase with Prostaglandin D Synthase Antibody (HY-P81994) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Prostaglandin D Synthase Antibody (HY-P81994) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using Prostaglandin D Synthase Antibody (YA1739). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81994, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium. -
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human prostate tissue using Prostaglandin D Synthase Antibody (YA1739). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81994, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium. -
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human glioma tissue using Prostaglandin D Synthase Antibody (YA1739). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81994, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium. -
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human gastric cancer tissue using Prostaglandin D Synthase Antibody (YA1739). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81994, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
Background
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Function
Prostaglandin D Synthase catalyzes PGH2 isomerization to PGD2, linking cyclooxygenase-derived prostanoid biosynthesis to sleep, pain, allergy, inflammation, and downstream PGJ2/PPARγ biology[1][2]. Mechanistically, PGD2 acts through DP and CRTH2 receptors, while PGDS enzymes connect arachidonic acid metabolism with central nervous system and immune-cell signaling[1][2]. In disease models, HPGDS augments inflammation followed by muscle injury, and HPGDS inhibition ameliorates muscular necrosis in mdx mice[3]. In Duchenne muscular dystrophy myocardium, HPGDS increases in mast cells and pericytes, supporting cell-type-focused PGD2 research in fibrotic cardiac specimens[4]. Compared with related isoforms, lipocalin-type PGDS and hematopoietic PGDS differ in amino acid sequence, tertiary structure, evolutionary origin, chromosomal and cellular localization, tissue distribution, and functional relevance[1]. Mouse knockout data further distinguish isoforms because L-Pgds deletion, but not H-Pgds deletion, elevates blood pressure and accelerates thrombogenesis[5]. For experimental applications, HQL-79 binds the human HPGDS catalytic cleft, while TAS-205 showed favorable safety in DMD patients and requires further efficacy evaluation[6][7].
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Subcellular Localization
Rough endoplasmic reticulum; Nucleus membrane; Golgi apparatus; Cytoplasm, perinuclear region; Secreted
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Expression
Tissue_specificity:Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes
Induction:By IL1B/interleukin-1 beta and thyroid hormone. Probably induced by dexamethasone, dihydrotestosterone (DHT) , progesterone, retinoic acid and retinal. Repressed by the Notch-Hes signaling pathway -
Subunit
Monomer
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SwissProt ID
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Synonyms
PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
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Research Field
Signal Transduction
Documentation
References
[1]. Urade Y, et al. Lipocalin-type and hematopoietic prostaglandin D synthases as a novel example of functional convergence. Prostaglandins Other Lipid Mediat. 2002 Aug;68-69:375-82. [Content Brief]
[2]. Smith WL, et al. Enzymes of the cyclooxygenase pathways of prostanoid biosynthesis. Chem Rev. 2011 Oct 12;111(10):5821-65. [Content Brief]
[3]. Mohri I, et al. Inhibition of prostaglandin D synthase suppresses muscular necrosis. Am J Pathol. 2009 May;174(5):1735-44. [Content Brief]
[4]. Hamamura K, et al. Hematopoietic Prostaglandin D Synthase Is Increased in Mast Cells and Pericytes in Autopsy Myocardial Specimens from Patients with Duchenne Muscular Dystrophy. Int J Mol Sci. 2024 Feb 3;25(3):1846. [Content Brief]
[5]. Song WL, et al. Lipocalin-Like Prostaglandin D Synthase but Not Hemopoietic Prostaglandin D Synthase Deletion Causes Hypertension and Accelerates Thrombogenesis in Mice. J Pharmacol Exp Ther. 2018 Dec;367(3):425-432. [Content Brief]
[6]. Aritake K, et al. Structural and functional characterization of HQL-79, an orally selective inhibitor of human hematopoietic prostaglandin D synthase. J Biol Chem. 2006 Jun 2;281(22):15277-86. [Content Brief]
[7]. Komaki H, et al. Early phase 2 trial of TAS-205 in patients with Duchenne muscular dystrophy. Ann Clin Transl Neurol. 2020 Feb;7(2):181-190. [Content Brief]
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