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SIL1 Antibody (YA7847)

(Synonyms: BAP; MSS; ULG5)
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Based on 1 Customer Validation

SIL1 Antibody (YA7847) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIL1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SIL1 Antibody (YA7847)
  • SIL1 Antibody (YA7847) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:50-500 1:100-250 1:100

Product Details

Description

SIL1 Antibody (YA7847) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIL1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 52.1 kDa
Immunogen

Full length human recombinant protein of human SIL1 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SIL1 Antibody (YA7847)
    Western blot analysis of extracts from Hela(lane 2(20μg) , K562(lane 3(20μg) ,HepG2(lane 4(20μg)and Mouse kidney tissue( lane 5(20μg) using SIL1 Antibody (HY-P88163). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    SIL1 is required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5

  • Subcellular Localization

    Endoplasmic reticulum lumen

  • Expression


    Tissue_Specificity: Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain

  • Isoforms & Post-Translational Modification

    Q9H173: 461 amino acids, molecular weight 52085 Da.

  • Subunit

    Interacts with HSPA5

  • SwissProt ID

    Q9H173

  • Synonyms

    BAP; MSS; ULG5

SIL1 Antibody (YA7847) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , K562(lane 3(20μg) ,HepG2(lane 4(20μg)and Mouse kidney tissue( lane 5(20μg) using SIL1 Antibody (HY-P88163). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • SIL1 Antibody (YA7847) Product Image
    1/16
  • Experimental Validation Results for SIL1 Antibody (YA7847)
  • SIL1 Antibody (YA7847) Product Image
  • Experimental Validation Results for SIL1 Antibody (YA7847)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , K562(lane 3(20μg) ,HepG2(lane 4(20μg)and Mouse kidney tissue( lane 5(20μg) using SIL1 Antibody (HY-P88163). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SIL1 Antibody (YA7847)
  • SIL1 Antibody (YA7847) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) , K562(lane 3(20μg) ,HepG2(lane 4(20μg)and Mouse kidney tissue( lane 5(20μg) using SIL1 Antibody (HY-P88163). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • SIL1 Antibody (YA7847) Product Image WB