HLA-DQA1 Antibody (YA8856)

Cat. No.: HY-P89408: Data Sheet COA
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HLA-DQA1 Antibody (YA8856) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HLA-DQA1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

HLA-DQA1 Antibody (YA8856) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HLA-DQA1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 28 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P01909

Gene ID

100133678 [NCBI]

Immunogen

Synthetic peptide within Human HLA-DQA1 aa 205-254 / 254.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1,000-1:5,000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
IP IP: Immunoprecipitation	1:10-1:50
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
mIHC mIHC: Multiplex Immunohistochemical	1:500-1:1,000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200
FC FC: Flow Cytometry	1:1,000

Sensitivity	Endogenous	Purity	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (25 μg) from Raji (lane 2), K562 (lane 3, Negative), Mouse spleen (lane 4), Rat lung (lane 5), Rat spleen (lane 6), and Rat skin (lane 7, Negative) using HLA-DQA1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Database

Entrez Gene: 100133678 Human ; 100507686 Human ; 100509457 Human ; 3117 Human ;

SwissProt: P01909 Human ;

OMIM: 146880 Human

Research Field

Immunology

Synonyms

HLA-DQA1; CD; CELIAC1; DQ-A1; GSE; HLA-DQA; DQ alpha 1 chain; DC-1 alpha chain; DC-alpha; HLA-DCA; MHC class II DQA1

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)