CD354 Antibody
(Synonyms: CD354, Triggering receptor expressed on myeloid cells 1, TREM-1, Triggering receptor expressed on monocytes 1, TREM1)CD354 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CD354.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
CD354 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CD354.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 25; 30 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 26 kDa
Synthetic peptide corresponding to the center region of human CD354.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD354 is a Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses. Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12. In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma. This cascade promotes the neutrophil- and macrophage-mediated release of pro-inflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections. By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Mostly expressed by immune cells of the myeloid lineage, such as monocytes, macrophages, neutrophils and dendritic cells. Expression is associated with a mature stage of myeloid development. Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Isoform 2 was detected in the lung, liver and mature monocytes.
Induction: Up-regulated by bacteria, fungi and bacterial lipopolysaccharides (LPS). -
Isoforms & Post-Translational Modification
CD354 has 3 isoforms, Q9NP99-1: amino acid length is 234, molecular weight is 26387 Da (predicted); Q9NP99-2: amino acid length is 150, molecular weight is 17563 Da (predicted); Q9NP99-3: amino acid length is 225, molecular weight is 25574 Da (predicted).可发生糖基化CD354 存在 3 个异构体，Q9NP99-1：氨基酸个数为 234 个，分子量为 26387 Da (预测)；Q9NP99-2：氨基酸个数为 150 个，分子量为 17563 Da (预测)；Q9NP99-3：氨基酸个数为 225 个，分子量为 25574 Da (预测)。Glycosylated
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Subunit
Monomer.
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SwissProt ID
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Synonyms
CD354, Triggering receptor expressed on myeloid cells 1, TREM-1, Triggering receptor expressed on monocytes 1, TREM1
Documentation
[1]. Bouchon A, et al. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000 May 15;164(10):4991-5. [Content Brief]
[2]. Arts RJ, et al. TREM-1 interaction with the LPS/TLR4 receptor complex. Eur Cytokine Netw. 2011 Mar;22(1):11-4. [Content Brief]
[3]. El Mezayen R, et al. Endogenous signals released from necrotic cells augment inflammatory responses to bacterial endotoxin. Immunol Lett. 2007 Jul 31;111(1):36-44. [Content Brief]
[4]. Read CB, et al. Cutting Edge: identification of neutrophil PGLYRP1 as a ligand for TREM-1. J Immunol. 2015 Feb 15;194(4):1417-21. [Content Brief]
[5]. Carrasco K, et al. TREM-1 multimerization is essential for its activation on monocytes and neutrophils. Cell Mol Immunol. 2019 May;16(5):460-472. [Content Brief]
[6]. Radaev S, et al. Crystal structure of the human myeloid cell activating receptor TREM-1. Structure. 2003 Dec;11(12):1527-35. [Content Brief]
[7]. Ormsby T, et al. Btk is a positive regulator in the TREM-1/DAP12 signaling pathway. Blood. 2011 Jul 28;118(4):936-45. [Content Brief]
[8]. Fortin CF, et al. Effects of TREM-1 activation in human neutrophils: activation of signaling pathways, recruitment into lipid rafts and association with TLR4. Int Immunol. 2007 Jan;19(1):41-50. [Content Brief]
[9]. Bouchon A, et al. TREM-1 amplifies inflammation and is a crucial mediator of septic shock. Nature. 2001 Apr 26;410(6832):1103-7. [Content Brief]
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