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NDUFS3 Antibody (YA6203)

(Synonyms: NDUFS3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3; mitochondrial; Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit)
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Based on 1 Customer Validation

NDUFS3 Antibody (YA6203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDUFS3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NDUFS3 Antibody (YA6203)
  • NDUFS3 Antibody (YA6203) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:500-1:2000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

NDUFS3 Antibody (YA6203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDUFS3.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 30 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NDUFS3 Antibody (YA6203)
    Western blot analysis of extracts from HEK293 (lane 1(40μg)) 、HepG2 (lane 2(40μg)) 、Mouse brain (lane 3(40μg)) 、Mouse heart (lane 4(40μg)) 、Rat brain (lane 5(40μg)) and Rat heart (lane 6(40μg)) using NDUFS3 Antibody (HY-P86511) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NDUFS3 core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I[1][2][3].

  • Subcellular Localization

    Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

  • Isoforms & Post-Translational Modification

    O75489 has 2 isomers: O75489-1: 30242 Da (predicted); O75489-2: 14679 Da (predicted).

  • Subunit

    Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits (PubMed:12611891). Interacts with NDUFAF3 (PubMed:19463981). Interacts with RAB5IF (PubMed:31536960). Found in subcomplexes containing subunits NDUFS2, MT-ND1 and NDUFA13 (PubMed:17209039, PubMed:18826940)

  • SwissProt ID

    O75489

  • Gene ID
    4722 [NCBI]
  • Synonyms

    NDUFS3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3; mitochondrial; Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit

NDUFS3 Antibody (YA6203) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(40μg)) 、HepG2 (lane 2(40μg)) 、Mouse brain (lane 3(40μg)) 、Mouse heart (lane 4(40μg)) 、Rat brain (lane 5(40μg)) and Rat heart (lane 6(40μg)) using NDUFS3 Antibody (HY-P86511) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NDUFS3 Antibody (YA6203) Product Image
    1/16
  • Experimental Validation Results for NDUFS3 Antibody (YA6203)
  • NDUFS3 Antibody (YA6203) Product Image
  • Experimental Validation Results for NDUFS3 Antibody (YA6203)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(40μg)) 、HepG2 (lane 2(40μg)) 、Mouse brain (lane 3(40μg)) 、Mouse heart (lane 4(40μg)) 、Rat brain (lane 5(40μg)) and Rat heart (lane 6(40μg)) using NDUFS3 Antibody (HY-P86511) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NDUFS3 Antibody (YA6203)
  • NDUFS3 Antibody (YA6203) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(40μg)) 、HepG2 (lane 2(40μg)) 、Mouse brain (lane 3(40μg)) 、Mouse heart (lane 4(40μg)) 、Rat brain (lane 5(40μg)) and Rat heart (lane 6(40μg)) using NDUFS3 Antibody (HY-P86511) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NDUFS3 Antibody (YA6203) Product Image WB