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GATAD2A Antibody (YA1966)

(Synonyms: GATAD2A; Hp66alpha; P66 alpha; p66alpha)
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Based on 1 Customer Validation

GATAD2A Antibody (YA1966) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GATAD2A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GATAD2A Antibody (YA1966)
  • GATAD2A Antibody (YA1966) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

GATAD2A Antibody (YA1966) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GATAD2A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 65,68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human p66 alpha aa175-236.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GATAD2A Antibody (YA1966)
    Western blot analysis of extracts from MCF-7 (lane2, 20μg), K562 (lane3, 20μg), HepG2 (lane4, 20μg) and Hela (lane5, 20μg) using GATAD2A Antibody (HY-P82221). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GATAD2A transcriptional repressor. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B[1][2][3][4].

  • Subcellular Localization

    Nucleus speckle; Nucleus; Chromosome

  • Expression


    Tissue_specificity:Ubiquitous, both in human tissues

  • Isoforms & Post-Translational Modification

    Q86YP4 has 3 isomers: Q86YP4-1: 68063 Da (predicted); Q86YP4-2: 65225 Da (predicted); Q86YP4-3: 68134 Da (predicted).

  • Subunit

    Homooligomer (PubMed:27732854). Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5 (PubMed:16428440, PubMed:27732854, PubMed:28977666, PubMed:33283408). The exact stoichiometry of the NuRD complex is unknown, and some subunits such as MBD2 and MBD3, GATAD2A and GATAD2B, and CHD3, CHD4 and CHD5 define mutually exclusive NuRD complexes (PubMed:16428440, PubMed:27732854, PubMed:28977666, PubMed:33283408). Component of the MeCP1 histone deacetylase complex (PubMed:21490301). Interacts with CDK2AP1 (PubMed:33283408). Interacts with CHD4 (PubMed:33283408). Interacts with ERCC6 (PubMed:26030138). Interacts with HDAC1 (PubMed:33283408). Interacts with HDAC2 (PubMed:33283408). Interacts with MBD2; this interaction is required for the enhancement of MBD2-mediated repression and for targeting to the chromatin (PubMed:12183469, PubMed:16415179, PubMed:21490301, PubMed:27732854, PubMed:33283408). Interacts with MBD3 (PubMed:12183469, PubMed:27732854). Interacts with MTA2 (PubMed:33283408). Interacts with ZMYND8 (PubMed:27732854, PubMed:35916866). Interacts with histone tails, including that of histones H2A, H2B, H3 and H4, the interaction is reduced by histone acetylation (PubMed:16415179)

  • SwissProt ID

    Q86YP4

  • Gene ID
    54815 [NCBI]
  • Synonyms

    GATAD2A; Hp66alpha; P66 alpha; p66alpha

  • Research Field

    -

GATAD2A Antibody (YA1966) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2, 20μg), K562 (lane3, 20μg), HepG2 (lane4, 20μg) and Hela (lane5, 20μg) using GATAD2A Antibody (HY-P82221). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • GATAD2A Antibody (YA1966) Product Image
    1/16
  • Experimental Validation Results for GATAD2A Antibody (YA1966)
  • GATAD2A Antibody (YA1966) Product Image
  • Experimental Validation Results for GATAD2A Antibody (YA1966)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2, 20μg), K562 (lane3, 20μg), HepG2 (lane4, 20μg) and Hela (lane5, 20μg) using GATAD2A Antibody (HY-P82221). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GATAD2A Antibody (YA1966)
  • GATAD2A Antibody (YA1966) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane2, 20μg), K562 (lane3, 20μg), HepG2 (lane4, 20μg) and Hela (lane5, 20μg) using GATAD2A Antibody (HY-P82221). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • GATAD2A Antibody (YA1966) Product Image WB