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MS2 Antibody (YA3247)

(Synonyms: MS2; CD156; CD156a)
Customer Review

Based on 1 Customer Validation

MS2 Antibody (YA3247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MS2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MS2 Antibody (YA3247)
  • Experimental Validation Results for MS2 Antibody (YA3247)
  • MS2 Antibody (YA3247) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

MS2 Antibody (YA3247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MS2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 89 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 89 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of MS2 aa71-83.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105612

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MS2 Antibody (YA3247)
    Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), RAW264.7 (lane 2, 15 μg), A431 (lane 3, 15 μg), K562 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using MS2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MS2 Antibody (YA3247)
    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using MS2 Antibody (HY-P83502) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    MS2 is a Possible involvement in extravasation of leukocytes

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Expressed on neutrophils and monocytes

  • Isoforms & Post-Translational Modification

    P78325 has 3 isomers: P78325-1: 88771 Da (predicted); P78325-2: 78850 Da (predicted); P78325-3: 80262 Da (predicted).

  • Subunit

    Interacts with FST3

  • SwissProt ID

    P78325

  • Gene ID
    101 [NCBI]
  • Synonyms

    MS2; CD156; CD156a

  • Research Field

    Cell Biology

MS2 Antibody (YA3247) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), RAW264.7 (lane 2, 15 μg), A431 (lane 3, 15 μg), K562 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using MS2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using MS2 Antibody (HY-P83502) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MS2 Antibody (YA3247) Product Image
    1/16
  • Experimental Validation Results for MS2 Antibody (YA3247)
  • Experimental Validation Results for MS2 Antibody (YA3247)
  • MS2 Antibody (YA3247) Product Image
  • Experimental Validation Results for MS2 Antibody (YA3247)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), RAW264.7 (lane 2, 15 μg), A431 (lane 3, 15 μg), K562 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using MS2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MS2 Antibody (YA3247)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using MS2 Antibody (HY-P83502) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MS2 Antibody (YA3247)
  • Experimental Validation Results for MS2 Antibody (YA3247)
  • MS2 Antibody (YA3247) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Raji (lane 1, 15 μg), RAW264.7 (lane 2, 15 μg), A431 (lane 3, 15 μg), K562 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using MS2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MS2 Antibody (YA3247) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using MS2 Antibody (HY-P83502) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MS2 Antibody (YA3247) Product Image WB
  • MS2 Antibody (YA3247) Product Image WB