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SLP2 Antibody (YA1720)

(Synonyms: stomatin like 2; SLP-2; HSPC108)
Customer Review

Based on 1 Customer Validation

SLP2 Antibody (YA1720) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • SLP2 Antibody (YA1720) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:200-1:500 1:200-1:1000 1:20-1:50

Product Details

Description

SLP2 Antibody (YA1720) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLP2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human STOML2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SLP2 Antibody (YA1720)
    Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), C6 (lane3, 20μg), HEK293 (lane4, 20μg) and Rat brain tissue (lane5, 20μg) using SLP2 Antibody (HY-P81975). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLP2 Antibody (YA1720)
    Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SLP2 Antibody (YA1720)
    Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    SLP2 is a Mitochondrial protein that probably regulates the biogenesis and the activity of mitochondria. Stimulates cardiolipin biosynthesis, binds cardiolipin-enriched membranes where it recruits and stabilizes some proteins including prohibitin and may therefore act in the organization of functional microdomains in mitochondrial membranes. Through regulation of the mitochondrial function may play a role into several biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis and cellular response to stress. May play a role in calcium homeostasis through negative regulation of calcium efflux from mitochondria. Required for mitochondrial hyperfusion a pro-survival cellular response to stress which results in increased ATP production by mitochondria. May also regulate the organization of functional domains at the plasma membrane and play a role in T-cell activation through association with the T-cell receptor signaling complex and its regulation

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Mitochondrion; Mitochondrion inner membrane; Lipid-anchor; Mitochondrion intermembrane space; Membrane raft; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Ubiquitously expressed at low levels. Expressed in lymphoid tissues (at protein level)

    Induction:Up-regulated in activated B- and T-cells and upon mitochondrial stress by chloramphenicol

  • Isoforms & Post-Translational Modification

    Q9UJZ1 has 2 isomers: Q9UJZ1-1: 38534 Da (predicted); Q9UJZ1-2: 33337 Da (predicted).
    Hyperphosphorylated at Ser-17 in some patients with monoclonal gammopathy of undetermined significance (MGUS), multiple myeloma (MM) and Waldenstrom macroglobulinemia due to impaired dephosphorylation by PP2A

  • Subunit

    Forms homooligomers. Interacts with MFN2; may form heterooligomers.

  • SwissProt ID

    Q9UJZ1

  • Gene ID
    30968 [NCBI]
  • Synonyms

    stomatin like 2; SLP-2; HSPC108

  • Research Field

    Cancer

SLP2 Antibody (YA1720) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), C6 (lane3, 20μg), HEK293 (lane4, 20μg) and Rat brain tissue (lane5, 20μg) using SLP2 Antibody (HY-P81975). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SLP2 Antibody (YA1720) Product Image
    1/16
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • SLP2 Antibody (YA1720) Product Image
  • Experimental Validation Results for SLP2 Antibody (YA1720)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), C6 (lane3, 20μg), HEK293 (lane4, 20μg) and Rat brain tissue (lane5, 20μg) using SLP2 Antibody (HY-P81975). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLP2 Antibody (YA1720)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SLP2 Antibody (YA1720)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • Experimental Validation Results for SLP2 Antibody (YA1720)
  • SLP2 Antibody (YA1720) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), C6 (lane3, 20μg), HEK293 (lane4, 20μg) and Rat brain tissue (lane5, 20μg) using SLP2 Antibody (HY-P81975). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • SLP2 Antibody (YA1720) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SLP2 Antibody (YA1720) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling SLP2 Antibody (HY-P81975, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SLP2 Antibody (YA1720) Product Image WB
  • SLP2 Antibody (YA1720) Product Image FC
  • SLP2 Antibody (YA1720) Product Image FC