RecA Antibody (YA6752)

Cat. No.: HY-P87059: Data Sheet COA
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RecA Antibody (YA6752) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RecA.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RecA Antibody (YA6752) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RecA.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 38 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Escherichia coli

SwissProt ID

P0A7G6

Gene ID

93779312 [NCBI]

Immunogen

Recombinant protein within becterium RecA aa 1-353.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352)|Mutations in this gene were selected in directed evolution experiments for resistance to intense ionizing radiation (3000 Gy)

Subcellular Localization：Cytoplasm

Expression：Induction: Induced by DNA damage, repressed by LexA (PubMed:10760155). Induced in response to low temperature (PubMed:8898389). Sensitive to temperature through changes in the linking number of the DNA. Induced by cold shock (42 to 15 degrees Celsius) (at protein level) (PubMed:8898389). 5.1-fold induced by hydroxyurea treatment (at protein level) (PubMed:20005847). mRNA levels are repressed in a mazE-mazF-mediated manner (PubMed:22412352)

Subunit：Polymerizes non-specifically on ssDNA to form filaments; filament formation requires ATP or ATP-gamma-S

Synonyms

DNA strand exchange and recombination protein with protease and nuclease activity antibody; lexB antibody; Protein recA antibody; recH antibody; Recombinase A antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)