RecA Antibody (YA6752)
(Synonyms: DNA strand exchange and recombination protein with protease and nuclease activity antibody; lexB antibody; Protein recA antibody; recH antibody; Recombinase A antibody; )Based on 1 publication(s) in Google Scholar
RecA Antibody (YA6752) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RecA.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Escherichia coli
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Publications Citing Use of MedChemExpress (MCE) RecA Antibody (YA6752)
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:2000 | 1:100 |
Product Details
RecA Antibody (YA6752) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RecA.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityEscherichia coli
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 38 kDa
Recombinant protein within becterium RecA aa 1-353.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Publications (1)
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Journal Impact Factor
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Most Recent
Background
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Function
RecA is required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA |Mutations in this gene were selected in directed evolution experiments for resistance to intense ionizing radiation (3000 Gy)[1].
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Subcellular Localization
Cytoplasm
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Expression
Induction: Induced by DNA damage, repressed by LexA (PubMed:10760155). Induced in response to low temperature (PubMed:8898389). Sensitive to temperature through changes in the linking number of the DNA. Induced by cold shock (42 to 15 degrees Celsius) (at protein level) (PubMed:8898389). 5.1-fold induced by hydroxyurea treatment (at protein level) (PubMed:20005847). mRNA levels are repressed in a mazE-mazF-mediated manner (PubMed:22412352)
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Subunit
Polymerizes non-specifically on ssDNA to form filaments; filament formation requires ATP or ATP-gamma-S
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SwissProt ID
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Synonyms
DNA strand exchange and recombination protein with protease and nuclease activity antibody; lexB antibody; Protein recA antibody; recH antibody; Recombinase A antibody;
Documentation
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Data Sheet (231 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)
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