GDF-8 Antibody (YA4877)

Cat. No.: HY-P85185: Data Sheet COA
SDS
User Guide for Antibodies
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GDF-8 Antibody (YA4877) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GDF-8.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

GDF-8 Antibody (YA4877) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GDF-8.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 43 kDa

Species Reactivity

Human

SwissProt ID

O14793

Gene ID

2660 [NCBI]

Immunogen

Purified recombinant fragment of GDF-8 N-terminal expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 2(20μg), HCT116 (lane 3(20μg), A375 (lane 4(20μg) and MCF-7 (lane 5(20μg) using GDF-8 Antibody (HY-P85185) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Acts specifically as a negative regulator of skeletal muscle growth

Subcellular Localization：Secreted

Subunit：Homodimer; disulfide-linked (PubMed:27625211). Interacts with WFIKKN2, leading to inhibit its activity (PubMed:12595574). Interacts with FST3 (PubMed:17878677)

Synonyms

MSTN; GDF8; Growth/differentiation factor 8; GDF-8; Myostatin

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mcf-10a-cell.html [Content Brief]