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GDF-8 Antibody (YA4877)

(Synonyms: MSTN; GDF8; Growth/differentiation factor 8; GDF-8; Myostatin)
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Based on 1 Customer Validation

GDF-8 Antibody (YA4877) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GDF-8.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GDF-8 Antibody (YA4877)
  • GDF-8 Antibody (YA4877) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10000

Product Details

Description

GDF-8 Antibody (YA4877) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GDF-8.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 43 kDa
Immunogen

Purified recombinant fragment of GDF-8 N-terminal expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GDF-8 Antibody (YA4877)
    Western blot analysis of extracts from HeLa (lane 2(20μg), HCT116 (lane 3(20μg), A375 (lane 4(20μg) and MCF-7 (lane 5(20μg) using GDF-8 Antibody (HY-P85185) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    GDF-8 acts specifically as a negative regulator of skeletal muscle growth

  • Subcellular Localization

    Secreted

  • Subunit

    Homodimer; disulfide-linked (PubMed:27625211). Interacts with WFIKKN2, leading to inhibit its activity (PubMed:12595574). Interacts with FST3 (PubMed:17878677)

  • SwissProt ID

    O14793

  • Gene ID
    2660 [NCBI]
  • Synonyms

    MSTN; GDF8; Growth/differentiation factor 8; GDF-8; Myostatin

GDF-8 Antibody (YA4877) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), HCT116 (lane 3(20μg), A375 (lane 4(20μg) and MCF-7 (lane 5(20μg) using GDF-8 Antibody (HY-P85185) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GDF-8 Antibody (YA4877) Product Image
    1/16
  • Experimental Validation Results for GDF-8 Antibody (YA4877)
  • GDF-8 Antibody (YA4877) Product Image
  • Experimental Validation Results for GDF-8 Antibody (YA4877)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), HCT116 (lane 3(20μg), A375 (lane 4(20μg) and MCF-7 (lane 5(20μg) using GDF-8 Antibody (HY-P85185) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GDF-8 Antibody (YA4877)
  • GDF-8 Antibody (YA4877) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 2(20μg), HCT116 (lane 3(20μg), A375 (lane 4(20μg) and MCF-7 (lane 5(20μg) using GDF-8 Antibody (HY-P85185) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GDF-8 Antibody (YA4877) Product Image WB