Frizzled 5 Antibody (YA4239)(PBS only)

Cat. No.: HY-P84542A: Data Sheet User Guide for Antibodies
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Frizzled 5 Antibody (YA4239) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Frizzled 5.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Frizzled 5 Antibody (YA4239) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Frizzled 5.

Host

Mouse

Molecular Weight

Predicted band size: 65 kDa;

Observed band size: 65-70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13467

Gene ID

7855 [NCBI]

Immunogen

Purified recombinant fragment of human FZD5 (AA:151-217) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor for Wnt proteins (PubMed:10097073, PubMed:20530549, PubMed:26908622, PubMed:9054360). Functions in the canonical Wnt/beta-catenin signaling pathway. In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling (By similarity). In neurons, activation by WNT7A promotes formation of synapses (PubMed:20530549). May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity). Plays a role in ocular development (PubMed:26908622)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Synapse; Perikaryon; Cell projection, dendrite; Cell projection, axon

Subunit：Binding of unsaturated fatty acid molecules (via FZ domain) promotes homodimerization (PubMed:28377511). Interacts with WNT2B (PubMed:12490564). Interacts with WNT3A (PubMed:26908622).

Synonyms

HFZ5; C2orf31; MGC129692; DKFZp434E2135

Documentation

User Guide for Antibodies (1077 KB)