Copy Link Print Information

Flag Tag Antibody (YA6859)

(Synonyms: FLAG Tag; flag)
Customer Review

Based on 1 Customer Validation

Flag Tag Antibody (YA6859) is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Flag Tag .

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Species independent

  • Formulation:

    1.Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
    2.Supplied in 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Flag Tag Antibody (YA6859)
  • Flag Tag Antibody (YA6859) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:10000 1:200-1:500 1:50-1:100 1:20-1:50 1:200-1:1000

Product Details

Description

Flag Tag Antibody (YA6859) is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Flag Tag .

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Species independent
  • Calculated Molecular Weight NA
Immunogen

A synthetic peptide of DDDDK tag

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719275

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
    2.Supplied in 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Flag Tag Antibody (YA6859)
    Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Flag Tag is a FLAG Tag is a multifunctional fusion tag for the purification of recombinant proteins. FLAG Tag maintances the natural folding of its fusing proteins. FLAG Tag can be removed by enterokinase, and eluted under non-denaturing conditions.

  • Synonyms

    FLAG Tag; flag

Flag Tag Antibody (YA6859) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Flag Tag Antibody (YA6859) Product Image
    1/16
  • Experimental Validation Results for Flag Tag Antibody (YA6859)
  • Flag Tag Antibody (YA6859) Product Image
  • Experimental Validation Results for Flag Tag Antibody (YA6859)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Flag Tag Antibody (YA6859)
  • Flag Tag Antibody (YA6859) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Flag Tag Antibody (YA6859) Product Image WB