Flag Tag Antibody (YA6859)

Cat. No.: HY-P87166: Data Sheet COA
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Flag Tag Antibody (YA6859) is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Flag Tag .

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Flag Tag Antibody (YA6859) is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to Flag Tag .

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Species Reactivity

Species independent

Immunogen

A synthetic peptide of DDDDK tag

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
IP IP: Immunoprecipitation	1:20
FC FC: Flow Cytometry	1:200-1:1000

Purity	Protein G affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：FLAG Tag is a multifunctional fusion tag for the purification of recombinant proteins. FLAG Tag maintances the natural folding of its fusing proteins. FLAG Tag can be removed by enterokinase, and eluted under non-denaturing conditions.

RRID

AB_3719275

Synonyms

FLAG Tag; flag

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)