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Relaxin 2 Antibody (YA1855)

(Synonyms: Prorelaxin H2)
Customer Review

Based on 1 Customer Validation

Relaxin 2 Antibody (YA1855) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Relaxin 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Relaxin 2 Antibody (YA1855) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Relaxin 2 Antibody (YA1855) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Relaxin 2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Relaxin 2 aa25-35.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104225

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Western blot analysis of extracts from HeLa(lane 2(20μg), 3T3(lane 3(20μg), C6(lane 4(20μg), MCF-7(lane 5(20μg) using Relaxin 2 Antibody (HY-P82110). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Immunohistochemical analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    Relaxin 2 is a Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Isoform 1 is expressed in the ovary during pregnancy. Also expressed in placenta, decidua and prostate. Isoform 2 is relatively abundant in placenta. It is in much lower abundance in the prostate gland. Not detected in the ovary

  • Isoforms & Post-Translational Modification

    P04090 has 2 isomers: P04090-1: 21043 Da (predicted); P04090-2: 12883 Da (predicted).

  • Subunit

    Heterodimer of a B chain and an A chain linked by two disulfide bonds

  • SwissProt ID

    P04090

  • Gene ID
    6013 [NCBI]
  • Synonyms

    Prorelaxin H2

  • Research Field

    Signal Transduction

Relaxin 2 Antibody (YA1855) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg), 3T3(lane 3(20μg), C6(lane 4(20μg), MCF-7(lane 5(20μg) using Relaxin 2 Antibody (HY-P82110). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Relaxin 2 Antibody (YA1855) Product Image
    1/16
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Relaxin 2 Antibody (YA1855) Product Image
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg), 3T3(lane 3(20μg), C6(lane 4(20μg), MCF-7(lane 5(20μg) using Relaxin 2 Antibody (HY-P82110). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Experimental Validation Results for Relaxin 2 Antibody (YA1855)
  • Relaxin 2 Antibody (YA1855) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg), 3T3(lane 3(20μg), C6(lane 4(20μg), MCF-7(lane 5(20μg) using Relaxin 2 Antibody (HY-P82110). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human brain tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver cancer tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Relaxin 2 Antibody (YA1855) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using Relaxin 2 Antibody (YA1855). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82110, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Relaxin 2 Antibody (YA1855) Product Image WB
  • Relaxin 2 Antibody (YA1855) Product Image IHC-P
  • Relaxin 2 Antibody (YA1855) Product Image IHC-P
  • Relaxin 2 Antibody (YA1855) Product Image IHC-P
  • Relaxin 2 Antibody (YA1855) Product Image IHC-P
  • Relaxin 2 Antibody (YA1855) Product Image mIHC
  • Relaxin 2 Antibody (YA1855) Product Image mIHC
  • Relaxin 2 Antibody (YA1855) Product Image mIHC
  • Relaxin 2 Antibody (YA1855) Product Image mIHC