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Cat. No.: HY-P87783

BRAT1 Antibody (YA7468)

BRAT1 Antibody (YA7468) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to BRAT1.

For research use only. We do not sell to patients.

Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	FC FC: Flow Cytometry
Dilution Ratio	1:1000-1:5000	1:100-1:500

Description

BRAT1 Antibody (YA7468) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to BRAT1.

Host Rabbit
Clonality Monoclonal,Recombinant
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 90 kDa

Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Appearance

Liquid
Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

BRAT1 component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex. Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex. Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer. In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress. Plays a role in regulating mitochondrial function and cell proliferation. Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors^[1]^[2]^[3]^[4]^[5]^[6].
Subcellular Localization

Nucleus,Cytoplasm
Expression

Tissue_Specificity: Ubiquitously expressed
Isoforms & Post-Translational Modification

Q6PJG6 has three isomers: Q6PJG6-1: 88119 Da (predicted); Q6PJG6-2: 30560 Da (predicted); Q6PJG6-3: 31676 Da (predicted).
Ubiquitinated by NEDD4, NEDD4L and ITCH; mono- and polyubiquitinated forms are detected
Subunit

Part of the multiprotein complex composed of BRAT1, WDR73, as well as integrator complex subunits INTS9 and INTS11 (PubMed:39032489)
SwissProt ID

Q6PJG6