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PFKP Antibody (YA1599)(PBS only)

(Synonyms: PFKP; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase)

PFKP Antibody (YA1599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PFKP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • PFKP Antibody (YA1599)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

PFKP Antibody (YA1599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PFKP.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 86 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 86 kDa
Immunogen

Recombinant protein of human PFKP aa540-751.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
    Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using PFKP Antibody (HY-P81854) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
    Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
    Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PFKP catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    Q01813 has 2 isomers: Q01813-1: 85596 Da (predicted); Q01813-2: 85316 Da (predicted).
    Phosphorylation at Ser-386 promotes interaction with ATG4B;GlcNAcylation decreases enzyme activity

  • Subunit

    Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet).

  • SwissProt ID

    Q01813

  • Gene ID
    5214 [NCBI]
  • Synonyms

    PFKP; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase

  • Research Field

    Signal Transduction

PFKP Antibody (YA1599)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using PFKP Antibody (HY-P81854) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PFKP Antibody (YA1599)(PBS only) Product Image
    1/16
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • PFKP Antibody (YA1599)(PBS only) Product Image
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using PFKP Antibody (HY-P81854) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • Experimental Validation Results for PFKP Antibody (YA1599)(PBS only)
  • PFKP Antibody (YA1599)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using PFKP Antibody (HY-P81854) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PFKP Antibody (YA1599)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PFKP Antibody (YA1599)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling PFKP with PFKP Antibody (HY-P81854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PFKP Antibody (HY-P81854) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PFKP Antibody (YA1599)(PBS only) Product Image WB
  • PFKP Antibody (YA1599)(PBS only) Product Image ICC
  • PFKP Antibody (YA1599)(PBS only) Product Image ICC