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LIM Kinase 2 Antibody (YA6637)

(Synonyms: LIM domain kinase 2 antibody; LIM kinase 2 antibody; LIMK 2 antibody; LIMK-2 antibody; Limk2 antibody; LIMK2_HUMAN antibody; )
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Based on 1 Customer Validation

LIM Kinase 2 Antibody (YA6637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LIM Kinase 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LIM Kinase 2 Antibody (YA6637)
  • LIM Kinase 2 Antibody (YA6637) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:50 1:1000 1-2μg/sample

Product Details

Description

LIM Kinase 2 Antibody (YA6637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LIM Kinase 2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 72 kDa
Immunogen

Recombinant protein within human LIMK2 aa 1-320.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LIM Kinase 2 Antibody (YA6637)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、NIH-3T3 (lane 2(20μg)) and PC-12 (lane 3(20μg)) using LIM Kinase 2 Antibody (HY-P86944) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    LIM Kinase 2 is a Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, cytoskeleton, spindle,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm,Nucleus,Cytoplasm,Cytoplasm, perinuclear region,Nucleus

  • Isoforms & Post-Translational Modification

    P53671 has three isomers: P53671-1: 72232 Da (predicted); P53671-2: 69904 Da (predicted); P53671-3: 77886 Da (predicted).
    Phosphorylated on serine and/or threonine residues by ROCK1

  • Subunit

    Interacts with LIMK2b

  • SwissProt ID

    P53671

  • Gene ID
    3985 [NCBI]
  • Synonyms

    LIM domain kinase 2 antibody; LIM kinase 2 antibody; LIMK 2 antibody; LIMK-2 antibody; Limk2 antibody; LIMK2_HUMAN antibody;

LIM Kinase 2 Antibody (YA6637) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、NIH-3T3 (lane 2(20μg)) and PC-12 (lane 3(20μg)) using LIM Kinase 2 Antibody (HY-P86944) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • LIM Kinase 2 Antibody (YA6637) Product Image
    1/16
  • Experimental Validation Results for LIM Kinase 2 Antibody (YA6637)
  • LIM Kinase 2 Antibody (YA6637) Product Image
  • Experimental Validation Results for LIM Kinase 2 Antibody (YA6637)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、NIH-3T3 (lane 2(20μg)) and PC-12 (lane 3(20μg)) using LIM Kinase 2 Antibody (HY-P86944) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LIM Kinase 2 Antibody (YA6637)
  • LIM Kinase 2 Antibody (YA6637) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、NIH-3T3 (lane 2(20μg)) and PC-12 (lane 3(20μg)) using LIM Kinase 2 Antibody (HY-P86944) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • LIM Kinase 2 Antibody (YA6637) Product Image WB