LIM Kinase 2 Antibody (YA6637)

Cat. No.: HY-P86944: Data Sheet COA
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LIM Kinase 2 Antibody (YA6637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LIM Kinase 2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

LIM Kinase 2 Antibody (YA6637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LIM Kinase 2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 72 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P53671

Gene ID

3985 [NCBI]

Immunogen

Recombinant protein within human LIMK2 aa 1-320.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、NIH-3T3 (lane 2(20μg)) and PC-12 (lane 3(20μg)) using LIM Kinase 2 Antibody (HY-P86944) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865)

Subcellular Localization：Cytoplasm, cytoskeleton, spindle,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm,Nucleus,Cytoplasm,Cytoplasm, perinuclear region,Nucleus

Isoforms & Post-Translational Modification：P53671 has three isomers: P53671-1: 72232 Da (predicted); P53671-2: 69904 Da (predicted); P53671-3: 77886 Da (predicted).
Phosphorylated on serine and/or threonine residues by ROCK1

Subunit：Interacts with LIMK2b

Synonyms

LIM domain kinase 2 antibody; LIM kinase 2 antibody; LIMK 2 antibody; LIMK-2 antibody; Limk2 antibody; LIMK2_HUMAN antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)