MDMX Antibody (YA4964)

Cat. No.: HY-P85272: Data Sheet COA
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MDMX Antibody (YA4964) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MDMX.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MDMX Antibody (YA4964) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MDMX.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 55kDa;

Species Reactivity

Human

SwissProt ID

O15151

Gene ID

4194 [NCBI]

Immunogen

Purified recombinant fragment of human MDM4 expressed in E. Coli. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Along with MDM2, contributes to TP53 regulation (PubMed:32300648). Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expressed in all tissues tested with high levels in thymus

Induction:Down-regulated by cisplatin (at protein level)

Isoforms & Post-Translational Modification：O15151 has 5 isomers: O15151-1: 54864 Da (predicted); O15151-2: 14185 Da (predicted); O15151-3: 13981 Da (predicted); O15151-4: 18195 Da (predicted); O15151-5: 49541 Da (predicted).
Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent;Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein

Subunit：Interacts with MDM2 (PubMed:16163388, PubMed:19838211, PubMed:32300648). Interacts with TP53, TP73 and USP2. Found in a trimeric complex with USP2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53

Synonyms

MDM4; MDMX; Protein Mdm4; Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4

Documentation

Select Batch:

Data Sheet (233 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)