MDM4 - MDM4 regulator of p53 Gene

Also Known as HDMX; MDMX; MRP1; BMFS6

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 4194

About MDM4

Cytogenetic location: 1q32.1 Genomic coordinates (GRCh38): 1:204,516,406-204,558,120 (from NCBI)

This gene has 16 transcripts (splice variants), 218 orthologues, 1 paralogue and is associated with 75 phenotypes. Ubiquitous expression in bone marrow (RPKM 9.7), skin (RPKM 6.5) and 24 other tissues.

Summary

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

MDM4 Products (7)

mRNA	Protein	Name
NM_001204171.2	NP_001191100.1	protein Mdm4 isoform 2
NM_001204172.2	NP_001191101.1	protein Mdm4 isoform 3
NM_001278516.2	NP_001265445.1	protein Mdm4 isoform 4
NM_001278517.2	NP_001265446.1	protein Mdm4 isoform 5
NM_001278518.2	NP_001265447.1	protein Mdm4 isoform 6
NM_001278519.2	NP_001265448.1	protein Mdm4 isoform 7
NM_002393.5	NP_002384.2	protein Mdm4 isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables enzyme binding	IPI IPI: Inferred from physical interaction	19838211	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	10469568	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA damage response, signal transduction by p53 class mediator	IEP IEP: Inferred from expression pattern	14660608	GOA
involved in cellular response to hypoxia	IEP IEP: Inferred from expression pattern	20810912	GOA
involved in negative regulation of DNA-templated transcription	IDA IDA: Inferred from direct assay	9271120	GOA
involved in negative regulation of protein catabolic process	IMP IMP: Inferred from mutant phenotype	10608892	GOA
involved in negative regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	9226370	GOA
involved in protein stabilization	IEP IEP: Inferred from expression pattern	10608892	GOA
involved in protein-containing complex assembly	IDA IDA: Inferred from direct assay	10608892	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of transcription repressor complex	IPI IPI: Inferred from physical interaction	8875929	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

MDM4 Protein Structure

SWIB

zf-RanBP

zf-C3HC4_3

0
100
200
300
400
490 a.a.

Protein Preferred Names	Protein Names
protein Mdm4	MDM4, p53 regulator

MDM4 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	MDM4	O15151	CAPN7	Homo sapiens	Q7Z479	Y2H Prey Pooling	25416956
Intra	MDM4	O15151	CSNK1A1	Homo sapiens	P48729	Anti Tag CoIP	33961781
Intra	MDM4	O15151	ZKSCAN3	Homo sapiens	Q9BRR0	Validated Y2H	32296183
Intra	MDM4	O15151	RNF115	Homo sapiens	Q9Y4L5	Validated Y2H	25416956
Intra	MDM4	O15151	MKRN3	Homo sapiens	Q13064	Validated Y2H	27107012
Intra	MDM4	O15151	MKRN3	Homo sapiens	Q13064	Validated Y2H	32296183
Intra	MDM4	O15151	USP7	Homo sapiens	Q93009	Anti Bait CoIP	15916963
Intra	MDM4	O15151	USP7	Homo sapiens	Q93009	Anti Tag CoIP	15916963
Intra	MDM4	O15151	USP7	Homo sapiens	Q93009	Pull Down	15916963
Intra	MDM4	O15151	YWHAZ	Homo sapiens	P63104	Anti Tag CoIP	28514442
Intra	MDM4	O15151	YWHAZ	Homo sapiens	P63104	Anti Tag CoIP	16511560
Intra	MDM4	O15151	UBE2D2	Homo sapiens	P62837	GMS	20705607
Intra	MDM4	O15151	UBE2D2	Homo sapiens	P62837	Ubiquitinase Assay	20705607
Intra	MDM4	O15151	YWHAE	Homo sapiens	P62258	Crosslink	36931259
Intra	MDM4	O15151	YWHAE	Homo sapiens	P62258	CoIP	18356162
Intra	MDM4	O15151	YWHAE	Homo sapiens	P62258	CoIP	16227609
Intra	MDM4	O15151	YWHAE	Homo sapiens	P62258	Anti Tag CoIP	16511560
Intra	MDM4	O15151	YWHAG	Homo sapiens	P61981	Anti Tag CoIP	16511572
Intra	MDM4	O15151	YWHAG	Homo sapiens	P61981	Anti Tag CoIP	16511560
Intra	MDM4	O15151	YWHAG	Homo sapiens	P61981	Anti Bait CoIP	16511572
Intra	MDM4	O15151	YWHAG	Homo sapiens	P61981	Pull Down	16511572
Intra	MDM4	O15151	TP53	Homo sapiens	P04637	ITC	19255450
Intra	MDM4	O15151	TP53	Homo sapiens	P04637	Anti Tag CoIP	33961781
Intra	MDM4	O15151	TP53	Homo sapiens	P04637	ITC	20515689
Intra	MDM4	O15151	TP53	Homo sapiens	P04637	Anti Bait CoIP	19521340
Intra	MDM4	O15151	TP53	Homo sapiens	P04637	Ubiquitinase Assay	20705607
Intra	MDM4	O15151	TP53	Homo sapiens	P04637	NMR	20515689
Intra	MDM4	O15151	MDM2	Homo sapiens	Q00987	Y2H	21988832
Intra	MDM4	O15151	MDM2	Homo sapiens	Q00987	Imaging	17159902
Intra	MDM4	O15151	MDM2	Homo sapiens	Q00987	Anti Bait CoIP	17159902
Intra	MDM4	O15151	MDM2	Homo sapiens	Q00987	Anti Tag CoIP	33961781
Intra	MDM4	O15151	MDM2	Homo sapiens	Q00987	CoIP	10608892
Intra	MDM4	O15151	MDM2	Homo sapiens	Q00987	Y2H Array	22493164
Intra	MDM4	O15151	MDM2	Homo sapiens	Q00987	Anti Tag CoIP	17159902
Intra	MDM4	O15151	CD160	Homo sapiens	O95971	Validated Y2H	32296183
Intra	MDM4	O15151	CD160	Homo sapiens	O95971	Y2H Array	25416956
Intra	MDM4	O15151	S100B	Homo sapiens	P04271	FPS	31837246
Intra	MDM4	O15151	RB1	Homo sapiens	P06400	Anti Tag CoIP	16510145
Intra	MDM4	O15151	RB1	Homo sapiens	P06400	Anti Bait CoIP	16510145
Intra	MDM4	O15151	WDR5	Homo sapiens	P61964	Validated Y2H	32296183
Intra	MDM4	O15151	S100A5	Homo sapiens	P33763	FPS	31837246
Intra	MDM4	O15151	S100A1	Homo sapiens	P23297	FPS	31837246
Intra	MDM4	O15151	NAA10	Homo sapiens	P41227	Validated Y2H	32296183
Intra	MDM4	O15151	S100A2	Homo sapiens	P29034	FPS	31837246
Intra	MDM4	O15151	BCL2	Homo sapiens	P10415	Anti Bait CoIP	19521340
Intra	MDM4	O15151	q3yba8_human	Homo sapiens	Q3YBA8	Anti Tag CoIP	16511560
Intra	MDM4	O15151	AIRIM	Homo sapiens	Q9NX04	Validated Y2H	32296183
Cross	MDM4	O15151	p03255-2	Human adenovirus C	P03255-2	Pull Down	15604276

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant MDM4 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P74756	MDM4/MDMX Protein, Human (His)	O15151-1/NP_002384.2 (M1-D134)	≥ 95%, as determined by reducing SDS-PAGE.

MDM4 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P85272	MDMX Antibody (YA4964)	WB, IHC-P, FC, ELISA	Human
HY-P85272A	MDMX Antibody (YA4964)(PBS only)	WB, IHC-P, FC, ELISA	Human

Related Diseases

Diseases	Alias
Bone Marrow Failure Syndrome 6	BMFS6
Intraocular Retinoblastoma	Pediatric Intraocular Retinoblastoma
Retinoblastoma	RB Trilateral Retinoblastoma RB1 Retinoblastoma, Trilateral Neuroblastoma Of Retina Rb - Retinoblastoma Eye Cancer, Retinoblastoma Retinal Cancer Retinal Tumor Glioma, Retinal Non-Hereditary Retinoblastoma Childhood Cancer Retinoblastoma Malignant Neoplasm Of Retina Retinal Neoplasms
Extraocular Retinoblastoma	Pediatric Extraocular Retinoblastoma
Bilateral Retinoblastoma
Unilateral Retinoblastoma
Leukocoria	Leucocoria
Glioblastoma	Glioblastoma Multiforme Gbm Adult Glioblastoma Multiforme Grade Iv Adult Astrocytic Tumor Primary Glioblastoma Multiforme Spongioblastoma Multiforme Adult Glioblastoma Primary Glioblastoma
Burkitt Lymphoma	Burkitt'S Lymphoma BL Burkitt Lymphoma, Somatic Burkitt Lymphoma/Leukaemia Burkitt'S Tumor Burkitt'S Tumor Or Lymphoma Malignant Lymphoma, Burkitt'S Type Small Non-Cleaved Cell Lymphoma, Burkitt'S Type Small Non-Cleaved Cell Lymphoma Burkitt Tumor Burkitts Lymphoma Lymphoma, Small Noncleaved-Cell Burkitt Tumour Diffuse Small Noncleaved Malignant Burkitt Lymphoma Malignant Burkitt Lymphoma “Burkitt-Like” Lymphoma Undifferentiated Burkitt Lymphoma Small Noncleaved Cell Burkitt Lymphoma
Li-Fraumeni Syndrome	Sarcoma Family Syndrome Of Li And Fraumeni Sbla Syndrome LFS Li-Fraumeni Familiar Cancer Susceptibility Syndrome Sarcoma, Breast, Leukaemia And Adrenal Gland Syndrome Lfs1 Li Fraumeni Syndrome Sarcoma, Breast, Leukemia, And Adrenal Gland Syndrome Lfl Sbla Syndrome Li-Fraumeni-Like Syndrome Li-Fraumeni Syndrome 1
Familial Retinoblastoma	Hereditary Retinoblastoma Retinoblastoma
Breast Cancer	Breast Carcinoma Male Breast Cancer Breast Cancer, Familial Malignant Neoplasm Of Breast Breast Cancer, Susceptibility To Breast Cancer, Early-Onset Malignant Tumor Of Breast Carcinoma Of Male Breast Breast Cancer, Invasive Ductal Breast Cancer, Protection Against Breast Cancer, Somatic Breast Cancer, Male Breast Cancer, Lobular, Somatic Breast Tumor Mammary Cancer Mammary Tumor Malignant Neoplasm Of Male Breast Mammary Carcinoma Male Breast Carcinoma Familial Cancer Of Breast Invasive Ductal Breast Carcinoma Breast Cancer Susceptibility Breast Cancer, Male, Susceptibility To Breast Cancer, Early-Onset, Susceptibility To Malignant Tumor Of The Breast Mammary Neoplasm Primary Breast Cancer Neoplasm Of Male Breast Carcinoma Of Breast Breast Cancer In Men Familial Breast Cancer Cancer Of Breast BC Breast Cancer Familial Breast Cancer Familial Male Breast Cancer, Familial Male Breast Male Carcinoma Breast Neoplasms Breast Neoplasms, Male Mammary Tumors Mammary Carcinomas Cancer, Breast Cancer, Breast, Susceptibility Invasive Breast Ductal Carcinoma Breast Neoplasm Susceptibility To Breast Cancer Mammary Neoplasms Animal Mammary Neoplasms Primary Malignant Neoplasm Of Breast Infiltrating Ductal Carcinoma Of Breast Infiltrating Duct Carcinoma Of Unspecified Site Infiltrating Ductular Carcinoma Of Unspecified Site Invasive Breast Carcinoma Of No Special Type Microinvasive Carcinoma Of Breast Carcinoma With Apocrine Differentiation

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Clinical Phase
Pre-clinical Phase

Clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-10412	CEP-1347		98.50%	Phase 3

Pre-clinical Phase

Bioactive Molecules (13)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-148409	MMRi62		99.59%	Unkown
HY-W097753	4-MDM		99.64%	Unkown
HY-123544	RDR03871	MDM2 Inhibitor	/	Unkown
HY-146806	YL93	Inhibitor	/	Unkown
HY-151169	MDM2/4-p53-IN-1	MDM2 Inhibitor	/	Unkown
HY-151170	MDM2/4-p53-IN-2		/	Unkown
HY-151171	MDM2/4-p53-IN-3	Inhibitor	/	Unkown
HY-183831	GPS167		/	Unkown
HY-RS08267	MDM4 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-158830B	FAM labled MDM4-targeting ASO sodium		/	Unkown
HY-158830C	Cy3 labled MDM4-targeting ASO sodium		/	Unkown
HY-RS16923	Mdm4 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS23366	Mdm4 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	MDM4	MGD	MGI:107934
Canis familiaris	MDM4	VGNC	VGNC:43107
Felis catus	MDM4	VGNC	VGNC:68224
Macaca mulatta	MDM4	VGNC	VGNC:74693
Rattus norvegicus	MDM4	RGD	RGD:1309306
Bos taurus	MDM4	VGNC	VGNC:31337
Others	MDM4	NCBI