C-erbB-2/HER2 Antibody (YA6411)

Cat. No.: HY-P86719: Data Sheet User Guide for Antibodies
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C-erbB-2/HER2 Antibody (YA6411) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to C-erbB-2/HER2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

C-erbB-2/HER2 Antibody (YA6411) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to C-erbB-2/HER2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 138 kDa

Species Reactivity

Human

SwissProt ID

P04626

Gene ID

2064 [NCBI]

Immunogen

Recombinant human HER2 protein aa full length

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:200-1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization|In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth

Subcellular Localization：Cell membrane,Cell projection, ruffle membrane,Cell membrane,Early endosome,Cytoplasm, perinuclear region,Nucleus,Cytoplasm,Nucleus,Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth

Isoforms & Post-Translational Modification：P04626 has 6 isomers: P04626-1: 137910 Da (predicted); P04626-2: 70917 Da (predicted); P04626-3: 62568 Da (predicted); P04626-4: 136501 Da (predicted); P04626-5: 134856 Da (predicted); P04626-6: 97382 Da (predicted).
Autophosphorylated

Subunit：Homodimer (PubMed:21454582)

Synonyms

Erb-B2 Receptor Tyrosine Kinase 2; NGL; NEU; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; EC 2.7.10.1; P185erbB2; MLN 19; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncoprotein 2; Neuroblastoma/Glioblastoma Derived Oncogene Homolog; Receptor Tyrosine-Protein Kinase ErbB-2; C-Erb B2/Neu Protein; CD340 Antigen; Herstatin; EC 2.7.10; CD340; HER-2; MLN19; TKR1;

Documentation

User Guide for Antibodies (1077 KB)