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  4. Monoclonal Antibodies Recombinant Antibodies
  5. Topoisomerase II alpha Antibody (YA3544)(PBS only)

Topoisomerase II alpha Antibody (YA3544)(PBS only)

製品番号: HY-P80980A
User Guide for Antibodies Technical Support

Topoisomerase II alpha Antibody (YA3544) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Topoisomerase II alpha.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Topoisomerase II alpha Antibody (YA3544) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Topoisomerase II alpha.
Host

Rabbit
分子量
Predicted band size: 174 kDa;
Observed band size: 174 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:20
純度 Protein A affinity purified. Conjugation Non-conjugated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity)
Subcellular Localization：Cytoplasm; Nucleus, nucleoplasm; Nucleus; Nucleus, nucleolus
Expression：
Tissue_specificity:This gene is expressed in the tonsils, spleen, lymph nodes, thymus, skin, pancreas, testes, colon, kidneys, liver, brain, and lungs (PubMed:9155056) . It is also found in high-grade lymphoma, squamous cell carcinoma of the lung, and seminoma (PubMed:9155056) .
Isoforms & Post-Translational Modification：P11388 has 4 isomers: P11388-1: 174385 Da (predicted); P11388-2: 177501 Da (predicted); P11388-3: 178712 Da (predicted); P11388-4: 182681 Da (predicted).
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation;(Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1; leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks;SUMOylated
Subunit：Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity (PubMed:18790802, PubMed:20457750). Interacts with DHX9; this interaction occurs in a E2 enzyme UBE2I- and RNA-dependent manner, negatively regulates DHX9-mediated double-stranded DNA and RNA duplex helicase activity and stimulates TOP2A-mediated supercoiled DNA relaxation activity (PubMed:12711669). Interacts with HNRNPU (via C-terminus); this interaction protects the topoisomerase TOP2A from degradation and positively regulates the relaxation of supercoiled DNA in a RNA-dependent manner (By similarity). Interacts with MCM3AP isoform GANP (PubMed:23652018). Interacts with ERCC6 (PubMed:26030138). Interacts with PLSCR1 (PubMed:17567603). Interacts with GCNA; this interaction allows the resolution of topoisomerase II (TOP2A) DNA-protein cross-links (By similarity). Interacts with POL1RA/RPA1 (via dock II) and UBTF in the context of Pol I complex; may assist Pol I transcription initiation by releasing supercoils occurring during DNA unwinding. Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405)
Synonyms
DNA topoisomerase II; DNA topoisomerase II-alpha; TOP2; TP2A; alpha isozyme, Topoisomerase II alpha
ドキュメンテーション
User Guide for Antibodies (1077 KB)

Topoisomerase II alpha Antibody (YA3544)(PBS only) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
Topoisomerase II alpha Antibody (YA3544)(PBS only)
製品番号:
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