CRHR1 Antibody
(Synonyms: CRFR, CRFR1, CRHR, CRHR1, Corticotropin-releasing factor receptor 1, CRF-R-1, CRF-R1, CRFR-1, Corticotropin-releasing hormone receptor 1, CRH-R-1, CRH-R1)CRHR1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CRHR1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
CRHR1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CRHR1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 66 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa
Synthetic peptide corresponding to the center region of human CRHR1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CRHR1 is a G protein-coupled receptor for CRH and UCN stress hormones, which plays a central role in whole body adaptation to stress. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and downstream effectors, such as adenylate cyclase. CRHR1 is mainly coupled to G(s) G protein (GNAS), mediating activation of adenylate cyclase activity and production of cAMP. In some conditions, can also couple to G(q) G protein (GNAQ) and mediate production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers. CRHR1-dependent signaling is a primary mediator of the neuroendocrine, autonomic (fight-or-flight) and behavioral responses to stress, acting as a key regulator of adaptation by activating the hypothalamus-pituitary-adrenal axis, leading to corticotropin hormone (ACTH) production (By similarity). Mediates both anxiogenic and anxiolytic response to stress in glutamatergic and dopaminergic neurons, respectively (By similarity). CRHR1 also plays a critical role in addiction by mediating the brain's stress response, particularly during withdrawal and relapse: it drives the transition to compulsive drug seeking by increasing anxiety and negative emotional states (By similarity)[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe. -
Isoforms & Post-Translational Modification
CRHR1 has 5 isoforms, P34998-2: amino acid length is 415, molecular weight is 47671 Da (predicted); P34998-1: amino acid length is 444, molecular weight is 50719 Da (predicted); P34998-3: amino acid length is 375, molecular weight is 43331 Da (predicted); P34998-4: amino acid length is 401, molecular weight is 46030 Da (predicted); P34998-5: amino acid length is 240, molecular weight is 28122 Da (predicted).
C-terminal Ser or Thr residues may be phosphorylated. Phosphorylation at Ser-301 by PKA prevents maximal coupling to Gq-protein, and thereby negatively regulates downstream signaling. -
Subunit
Heterodimer; heterodimerizes with GPER1 (By similarity).
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SwissProt ID
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Synonyms
CRFR, CRFR1, CRHR, CRHR1, Corticotropin-releasing factor receptor 1, CRF-R-1, CRF-R1, CRFR-1, Corticotropin-releasing hormone receptor 1, CRH-R-1, CRH-R1
Documentation
[1]. Tao J, et al. Activation of corticotropin-releasing factor receptor 1 selectively inhibits CaV3.2 T-type calcium channels. Mol Pharmacol. 2008 Jun;73(6):1596-609. [Content Brief]
[2]. Pioszak AA, et al. Molecular recognition of corticotropin-releasing factor by its G-protein-coupled receptor CRFR1. J Biol Chem. 2008 Nov 21;283(47):32900-12. [Content Brief]
[3]. Pal K, et al. Structural basis for hormone recognition by the Human CRFR2{alpha} G protein-coupled receptor. J Biol Chem. 2010 Dec 17;285(51):40351-61. [Content Brief]
[4]. Dunn HA, et al. Role of SAP97 protein in the regulation of corticotropin-releasing factor receptor 1 endocytosis and extracellular signal-regulated kinase 1/2 signaling. J Biol Chem. 2013 May 24;288(21):15023-34. [Content Brief]
[5]. Hollenstein K, et al. Structure of class B GPCR corticotropin-releasing factor receptor 1. Nature. 2013 Jul 25;499(7459):438-43. [Content Brief]
[6]. Coin I, et al. Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR. Cell. 2013 Dec 5;155(6):1258-69. [Content Brief]
[7]. Liang YL, et al. Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation. Mol Cell. 2020 Feb 6;77(3):656-668.e5. [Content Brief]
[8]. Ma S, et al. Molecular Basis for Hormone Recognition and Activation of Corticotropin-Releasing Factor Receptors. Mol Cell. 2020 Feb 6;77(3):669-680.e4. [Content Brief]
[9]. Papadopoulou N, et al. Protein kinase A-induced negative regulation of the corticotropin-releasing hormone R1alpha receptor-extracellularly regulated kinase signal transduction pathway: the critical role of Ser301 for signaling switch and selectivity. Mol Endocrinol. 2004 Mar;18(3):624-39. [Content Brief]
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