Copy Link Print Information

Snrp116 Antibody (YA5538)

(Synonyms: EFTUD2; KIAA0031; SNRP116; 116 kDa U5 small nuclear ribonucleoprotein component; Elongation factor Tu GTP-binding domain-containing protein 2; SNU114 homolog; hSNU114; U5 snRNP-specific protein; 116 kDa; U5-116 kDa)
Customer Review

Based on 1 Customer Validation

Snrp116 Antibody (YA5538) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Snrp116.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Snrp116 Antibody (YA5538)
  • Snrp116 Antibody (YA5538) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

Snrp116 Antibody (YA5538) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Snrp116.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 109 kDa
Immunogen

Synthesized peptide derived from human EFTUD2. AA range:300-400

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Snrp116 Antibody (YA5538)
    Western blot analysis was performed on nuclear protein extracts (25 μg) from HeLa (lane 2) using Snrp116 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Snrp116 is required for pre-mRNA splicing as component of the spliceosome, including pre-catalytic, catalytic and post-catalytic spliceosomal complexes. Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable)[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Q15029 has 3 isomers: Q15029-1: 109436 Da (predicted); Q15029-2: 105384 Da (predicted); Q15029-3: 108283 Da (predicted).

  • Subunit

    Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome (PubMed:16723661, PubMed:26912367).

  • SwissProt ID

    Q15029

  • Gene ID
    9343 [NCBI]
  • Synonyms

    EFTUD2; KIAA0031; SNRP116; 116 kDa U5 small nuclear ribonucleoprotein component; Elongation factor Tu GTP-binding domain-containing protein 2; SNU114 homolog; hSNU114; U5 snRNP-specific protein; 116 kDa; U5-116 kDa

References

[1]. Oka Y, et al. UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells. EMBO Rep. 2014 Sep;15(9):956-64. [Content Brief]

[2]. Bertram K, et al. Cryo-EM structure of a human spliceosome activated for step 2 of splicing. Nature. 2017 Feb 16;542(7641):318-323. [Content Brief]

[3]. Zhang X, et al. An Atomic Structure of the Human Spliceosome. Cell. 2017 May 18;169(5):918-929.e14. [Content Brief]

[4]. Bertram K, et al. Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation. Cell. 2017 Aug 10;170(4):701-713.e11. [Content Brief]

[5]. Zhan X, et al. Structure of a human catalytic step I spliceosome. Science. 2018 Feb 2;359(6375):537-545. [Content Brief]

[6]. Zhang X, et al. Structure of the human activated spliceosome in three conformational states. Cell Res. 2018 Mar;28(3):307-322. [Content Brief]

[7]. Haselbach D, et al. Structure and Conformational Dynamics of the Human Spliceosomal B(act) Complex. Cell. 2018 Jan 25;172(3):454-464.e11. [Content Brief]

[8]. Zhan X, et al. Structures of the human pre-catalytic spliceosome and its precursor spliceosome. Cell Res. 2018 Dec;28(12):1129-1140. [Content Brief]

[9]. Fica SM, et al. A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation. Science. 2019 Feb 15;363(6428):710-714. [Content Brief]

[10]. Liu S, et al. The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP. RNA. 2006 Jul;12(7):1418-30. [Content Brief]

View More

Snrp116 Antibody (YA5538) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on nuclear protein extracts (25 μg) from HeLa (lane 2) using Snrp116 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Snrp116 Antibody (YA5538) Product Image
    1/16
  • Experimental Validation Results for Snrp116 Antibody (YA5538)
  • Snrp116 Antibody (YA5538) Product Image
  • Experimental Validation Results for Snrp116 Antibody (YA5538)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on nuclear protein extracts (25 μg) from HeLa (lane 2) using Snrp116 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Snrp116 Antibody (YA5538)
  • Snrp116 Antibody (YA5538) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on nuclear protein extracts (25 μg) from HeLa (lane 2) using Snrp116 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Snrp116 Antibody (YA5538) Product Image WB